中国临床药理学杂志2018，Vol.34Issue(7)：809-811,3.DOI:10.13699/j.cnki.1001-6821.2018.07.022
微RNA-196a和微RNA-210在胰腺癌中的表达及其与生存时间的关系
Expressions of microRNA-196a and microRNA-210 in pancreatic cancer and their relationship with survival time
摘要
Abstract
Objective To analyze the expressions of microRNA-196a (miR-196a) and microRNA-210 (miR-210) in pancreatic cancer and their relationship with the survival time.Methods Twenty-six cases of pancreatic cancer (A group),20 patients with benign pancreatic disease (B group) and 24 healthy subjects (C group) were selected as the subjects.The expressions of miR-196a and miR-210 in the serum of 3 groups of patients were detected by real time fluorescence real time fluorescence quantitative (qRT-PCR) method,and the survival time of the patients with pancreatic cancer was observed.The relationship between the expression of miR-196a and miR-210 in 3 groups,the levels of miR-196a and miR-210 and the survival time of the patients with pancreatic cancer were analyzed.Results The levels of miR-196a expression in A,B and C groups was (1.79 ±0.95),(1.24 ±0.92) and (0.71 ±0.68),respectively,and the miR -210 expression levels were (2.32 ±0.54),(1.25 ±0.36) and (0.98 ±0.22).There were significant differences between A group and B,C groups (all P ＜ 0.05).The half year survival rate of the patients with pancreatic cancer was 61.54％,the 1 year survival rate was 46.15％,and the 2 year survival rate was 3.85％.For patients with pancreatic cancer,the survival time in miR-196a high and low expression groups was(7.58 ± 3.43) and (12.38 ±5.17) months,respectively,in miR-210 high expression group and low expression group was (6.92 ± 3.01) and (10.88 ±4.28) months,the differences were statistically significant (P ＜ 0.05).The sensitivity,specificity and accuracy of miR-196a were 76.92％,93.18％ and 70.10％,which of miR-210 68.00％,93.18％ and 61.18％,of joint detection 88.46％,95.45％ and 83.92％.The indexes obtained by combined detection had statistically significant differences compared with those detected separately (all P ＜ 0.01) Conclusion The expressions of miR-196a and miR-210 are specifically expressed in serum of patients with pancreatic cancer.The combined detection can improve the diagnostic accuracy of pancreatic cancer,and the expression levels of miR-196a and miR-210 are related to the survival time of pancreatic cancer patients.关键词
胰腺癌/微RNA-196a/微RNA-210/生存时间Key words
pancreatic cancer/microRNA-196a/microRNA-210/survival time分类
医药卫生引用本文复制引用
崔大鹏,张迎春,连晶晶,刘博,刘斐,韩磊,王玉佳,高树全..微RNA-196a和微RNA-210在胰腺癌中的表达及其与生存时间的关系[J].中国临床药理学杂志,2018,34(7):809-811,3.基金项目
河北省医学科学研究重点课题计划基金资助项目(20170819) （20170819）
