中国临床药理学杂志2018，Vol.34Issue(7)：824-826,3.DOI:10.13699/j.cnki.1001-6821.2018.07.027
四逆散对创伤后应激障碍大鼠海马神经元信号强度的影响
Effect of Sinisan on hippocampal neurons signal intensity in rats with post-traumatic stress disorder
摘要
Abstract
Objective To study the influence of Sinisan on hippocampal CA1 and CA3 neuron spatiotemporal characteristics in rats with post-traumatic stress disorder(PTSD).Methods SD rats were divided into five groups:normal group,blank group,model group,control group,experimental group.Except for normal group and blank group,the rats in other groups were stimulated by the current stimulation plus dark claustrophobic method to replicate the posttraumatic stress disorder (PTSD) model.The blank group and model group were given 0.9％ NaCl 10 mL · kg-1 at 1 h before making the model.The control group and experimental group was given paroxetine hydrochloride 0.42 mg · mL-1 and Sinisan(containing crude drug 0.24 g · mL-1) by intragastric administration,respectively.The model replicated 7 d,and each electric shock was 30 min every morning and afternoon.The neural coding on amplitude in hippocampal CA1 and CA3 were detected by the M-NEMEA after administration,and the spectral density spectrum was obtained from Neuro explorer neural data analysis software.Results The power spectral densities of CA1 and CA3 in hippocampus of rats were determined,they were (-109.08 ± 15.41) and (-108.46 ± 13.16)dB in normal group,(-110.17 ± 14.30)and (-109.54 ± 14.16)dB in blank group,and the differences were not statistically significant (all P ＞ 0.05).Compared with the blank group,the power spectral densities of hippocampus in the model group decreased significantly,which were (-117.39 ± 14.97),(-122.51 ± 16.42) dB,and the differences were statistically significant (all P ＜0.01).Compared with the model group,the power spectral densities of the experimental group in hippocampus were significantly higher (P＜0.01),which were (-110.83 ±11.40),(-110.36 ±10.87)dB.Conclusion The Sinisanhas significant positive effect on hippocampal neuron signal intensity.关键词
四逆散/创伤后应激障碍/海马区/功率谱密度Key words
Sinisan/post-traumatic stress disorder/hippocampus/power spectral density分类
医药卫生引用本文复制引用
李越峰,边甜甜,牛江涛,曹瑞,张泽国,司昕蕾,严兴科..四逆散对创伤后应激障碍大鼠海马神经元信号强度的影响[J].中国临床药理学杂志,2018,34(7):824-826,3.基金项目
国家自然科学基金资助项目(81460611) （81460611）
教育部科学技术研究重点基金资助项目(212186) （212186）
甘肃省自然科学基金资助项目(2010:1010RJZA212 （）
2014:145RJZA076) （）
甘肃省财政厅高校基本科研业务费专项基金资金项目(2013-2) （2013-2）
兰州市科技计划基金资金项目(2014-1-188) （2014-1-188）
甘肃省中医药管理局科研课题基金资金项目(GZK-2015-57) （GZK-2015-57）
