中国临床药理学杂志2018，Vol.34Issue(7)：827-830,4.DOI:10.13699/j.cnki.1001-6821.2018.07.028
芍药总苷对6MV-X射线放射性食管炎模型大鼠的作用研究
Effect of total glucosides of peony on esophagitis model of rats by 6MV-X ray radiation
摘要
Abstract
Objective To analyze the effect of total glucosides of peony (TGP) on nerve growth factor(NGF) and its receptor in 6MV-X ray radiation induced esophagitis model rats.Methods The male Wistar rats were selected,and radiation esophagitis rat model were established by 6MV-X ray single irradiation,the model rats were randomly divided into model group,control group,experimental-L group,experimental-H group,each with 17 rats;another 17 rats in blank group did not receive radiation.The model group and blank group were treated with sodium chloride injection 2 mL,tid;the control group was treated with western medicine mixture (sodium chloride injection 250 mL + lidocaine 3.2 mL + dexamethasone 1.6 mg + gentamicin sulphate 51.2 × 104 u) 2 mL,tid;the experimental-L group and experimental-H group were treated with 2,6 mg · mL-1 TGP solution 2 mL,tid.All rats were given the drug continuously for 8 days.The score of pathological changes of esophagus were observed by optical microscope.The serum levels of type Ⅲ procollagen (PC Ⅲ) and transforming growth factor β1 (TGF-β1) were detected by enzyme-linked immunosorbent assay.The expression levels of nerve growth factor(NGF),tyrosine protein kinase A (TrkA)protein,P75 protein were detected by Western blotting.Results After treatment,the score of pathological changes of esophagus in blank group,model group,corntrol group,experimental-L group,experimental-H group were respectively 0,(4.24 ± 0.58),(2.15 ± 0.26),(1.08 ± 0.15) and (0.53 ± 0.07) point,serum PC Ⅲ levels in the 5 groups were respectively (40.73 ± 5.24),(90.48 ± 10.38),(71.23 ± 8.72),(58.46 ± 7.66) and (49.36-± 6.12) μg · L-1,the expression levels of NGF in esophageal tissue in the 5 groups were respectively 0.54 ± 0.07,1.32 ± 0.18,1.13 ± 0.12,0.84 ± 0.11 and 0.69 ± 0.08,there were significant differences between groups (all P ＜ 0.05).Conclusion TGP can reduce the pathological damage degree of 6MV-X ray radiation induced esophagitis model rats,reduce serum PC Ⅲ and TGF-β1 levels,prevent and cure esophageal fibrosis,which may be related to decrease the expression levels of NGF and its receptor TrkA protein and P75 protein.关键词
芍药总苷/放射性食管炎/神经生长因子/酪氨酸蛋白激酶Key words
total glucosides of peony/radiation esophagitis/nerve growth factor/tyrosine protein kinase分类
医药卫生引用本文复制引用
汪治宇,郭盛虎,李幸,曹静,王聪,韩雪..芍药总苷对6MV-X射线放射性食管炎模型大鼠的作用研究[J].中国临床药理学杂志,2018,34(7):827-830,4.基金项目
河北省自然科学基金资助项目(H2015206222) （H2015206222）
