中国临床药理学杂志2018，Vol.34Issue(7)：841-844,4.DOI:10.13699/j.cnki.1001-6821.2018.07.032
天花粉对人乳腺癌MCF-7细胞凋亡及相关基因表达的影响
Effect of trichosanthin on the apoptosis and the expression of related gene in human breast cancer MCF-7 cell
摘要
Abstract
Objective To explore the effect of trichosanthin on the apoptosis and the expression of related gene in human breast cancer MCF -7 cell.Methods Logarithmic phase human breast cancer MCF-7 cell managed by 30,60,90,120 μg · mL-1 trichosanthin and acted as test group,control group managed by equivalent 0.9％ NaCl,cultured for 24,48,72 h.The changes of cell morphology and nucleus apoptosis morphology were compared,the proliferation inhibition of human breast cancer MCF-7 cell was detected by methyl thiazolyl tetrazolium (MTT),the effect of trichosanthin on the expression of cysteinyl aspartate specific proteinase(caspase)-3 and caspase-8 of human breast cancer MCF-7 cell was deteted by spectrophotometry.Results The cell morphology and nucleus apoptosis morphology of human breast cancer MCF-7 cell in test group was obvious;and the changes of nucleus apoptosis morphology were more obvious with the increasing of the trichosanthin action concentration.The proliferation inhibition rates in 30,60,90,120 μg · mL-1test group at 24 h after managed were (1.61 ±0.94) ％,(2.81 ±1.01) ％,(7.05 ±1.03) ％,(9.59±1.12)％.The proliferation inhibition rates at 48 h after managed were (2.13 t± 1.01) ％,(6.14 ± 1.12) ％,(9.05 ± 1.09) ％,(19.60 ± 1.15) ％.The proliferation inhibition rates at 72 h after managed were (3.28 ± 1.07) ％,(7.18 ± 1.51) ％,(18.39 ± 1.81)％,(29.59 ± 1.63) ％.The proliferation inhibition rates in test group increased with the increasing of the trichosanthin action concentration and the extension of trichosanthin action time (P ＜0.01).The caspase-3 of 90 μg · mL-1 test group at 24,48,72 h were 1.49 ±0.15,2.36 ±0.25,2.15 ±0.23;and the caspase-8 were 1.94 ±0.26,1.56 ± 0.19,1.39 ± 0.20.The caspase-3 and caspase-8 of human breast cancer MCF-7 cell in control group had no significant changes with the extension of trichosanthin action time (P ＞ 0.05),and the caspase-3 in 90 μg · mL-1 test group increased at first and then decreased (P ＜0.05 or P＜0.01),and which at 48 h was highest.The caspase-8 decreased gradually (P ＜ 0.01);the caspase-3 and caspase-8 in 90 μg · mL-1 test group at each time were higher than that in control group (P ＜ 0.01).Conclusion Trichosanthin can promote the apoptosis of human breast cancer MCF-7 cell effectively and inhibit the proliferation,and proliferation inhibition is time-dose dependent,the action mechanism maybe related to the up-regulation of the expression of caspase-3 and caspase-8 in human breast cancer MCF-7 cell.关键词
乳腺癌/人乳腺癌MCF-7细胞/天花粉/细胞凋亡/胱天蛋白酶-3Key words
breast cancer/human breast cancer MCF-7 cell/trichosanthin/cell apoptosis/cysteinyl aspartate specific proteinase-3分类
医药卫生引用本文复制引用
刘翔..天花粉对人乳腺癌MCF-7细胞凋亡及相关基因表达的影响[J].中国临床药理学杂志,2018,34(7):841-844,4.基金项目
陕西省科技计划基金资助项目(2013JM2004) （2013JM2004）
