中国临床药理学杂志2018，Vol.34Issue(7)：845-847,3.DOI:10.13699/j.cnki.1001-6821.2018.07.033
细胞外基质金属蛋白酶诱导因子对人卵巢癌SKOV3细胞凋亡和迁移侵袭的影响
Influence of extracellular matrix metalloproteinase inducer on the apoptosis,migration and invasion abilities of human ovarian cancer SKOV3 cells
摘要
Abstract
Objective To investigate the roles of icariin combining extracellular matrix metalloproteinase inducer (EMMPRIN) in apoptosis,migration and invasion abilities of human ovarian cancer SKOV3 cells.Methods SKOV3 cells with stably expressed EMMPRIN were established as the test group,SKOV3 cells transfected with pcDNA and SKOV3 cells were the control group and the blank group,respectively.Flow cytometry was performed to measure the apoptosis rate,scratch and Transwell assays were used to detect the migration and invasion abilities.Western blot assay was performed to analyze the expression levels of EMMPRIN,Bcl-2 and Bax.Results The main indexes in blank,control and test groups were compared:apoptosis rates were (10.27 ± 1.35) ％,(12.40 ±0.70)％ and (5.67 ±0.96)％,average migration indexes were (0.25 ± 0.03),(0.64 ± 0.03) and (O.83 ± 0.02),average number of invasive cells were (45.75 ± 2.87),(120.00 ±3.92) and (153.25 ±8.06),expression of EMMPRIN were (0.12±0.02),(0.22 s±0.01) and (0.34 ±0.02),expression of Bcl-2 were (0.06 ±0.01),(0.55 ±0.02) and (0.87 ±0.03),expression of Bax were (0.37 ± 0.02),(0.28 ± 0.01) and (0.12 ± 0.01).The differences were statistically significant between test group and blank,control groups (P ＜ 0.001,P ＜ 0.01).Conclusion Overexpression of EMMPRIN inhibits apoptosis and promotes migration and invasion abilities of human ovarian cancer SKOV3 cells.关键词
细胞外基质金属蛋白酶诱导因子/卵巢癌/细胞凋亡/迁移侵袭Key words
extracellular matrix metalloproteinase inducer/ovarian cancer/apoptosis/migration and invasion分类
医药卫生引用本文复制引用
蒋绍艳,常宏,邓少洁,樊丹怡..细胞外基质金属蛋白酶诱导因子对人卵巢癌SKOV3细胞凋亡和迁移侵袭的影响[J].中国临床药理学杂志,2018,34(7):845-847,3.基金项目
深圳市科技研发资金基础研究基金资助项目(20160427191320225) （20160427191320225）
