中国临床药理学杂志2018，Vol.34Issue(8)：949-952,4.DOI:10.13699/j.cnki.1001-6821.2018.08.010
MS23对糖尿病大鼠冠状动脉肌原性反应和电位依赖性钾通道的影响
Effects of MS23 on the vascular myogenic responses and the myocyte voltage-gated K+ channel currents of diabetic rat coronary artery
摘要
Abstract
Objective To study the effects of a new phosphodiesterase inhibitor MS23 on the myogenic responses of diabetic rat coronary artery (RCA) and the myocyte potassium currents via the voltage-gated potassium channels (Kv).Methods Rats were rendered diabetic with a peritoneal injection of streptozocin at 60 mg · kg-1.At the 4th week after treatment,the diabetic rats were randomly divided into model group,low-dose,and high-dose groups of MS23 (0.3 mg · kg-1 and 1.0 mg · kg-1 orally administrated once a day for 8 weeks),while the normal rats were set as the control group.Model group and control group were fed with 0.9％ NaCl of equal volume once a day from the fourth week to the twelfth week.A wire myograph was used to observe the myogenic effects of isolated RCA.The Kv currents of single rat coronary arterial smooth muscle cell (RCASMC) were studied with patch clamp.Results After administration,the maximum vasorelaxations of RCA to acetylcholine in control group,model group,low-dose,high-dose group were (74.45 ± 6.30) ％,(56.84 ± 5.51) ％,(77.52 ± 5.27) ％,(86.33 ± 8.88) ％,respectively.The maximum vasorelaxations to sodium nitroprusside in the four groups were (88.50 ± 5.81)％,(56.65 ±8.62)％,(80.50 ±7.85)％,(91.60 ±5.97)％,respectively.The maximum RCASMC Kv current density in the four groups were (53.69±4.02) pA · pF-1,(28.25±3.69) pA · pF-1,(38.78±4.55) pA · pF-1,(63.35 ± 4.57) pA · pF-1,respectively.There were significant differences between the model group and the blank group/low-dose group/high-dose group (P ＜ 0.05).The maximum vasorelaxations of diabetic RCA to MS23 (30 μmol · L-1) was (70.69 ± 12.72)％,and preincubation with Kv inhibitor 4-AP (3 mmol · L-1) reduced the MS23-induced maximum relaxation to (34.47 ± 10.08) ％,with significant difference (P ＜ 0.05).Conclusion MS23 relaxes diabetic rat coronary artery and enhances diabetic RCASMC Kv currents.关键词
MS23/冠状动脉/糖尿病/血管舒张/电位依赖性钾通道Key words
MS23/coronary artery/diabetes/vasorelaxation/voltage-gated potassium channel分类
医药卫生引用本文复制引用
刘宇,王燕,宋奇颖,郭鹏美,牛龙刚,张明升..MS23对糖尿病大鼠冠状动脉肌原性反应和电位依赖性钾通道的影响[J].中国临床药理学杂志,2018,34(8):949-952,4.基金项目
国家自然科学基金资助项目(81603111) （81603111）
山西省“1331工程”重占学科建设计划基金资助项目(晋教科(2017)6号) （晋教科(2017）
