中国临床药理学杂志2018，Vol.34Issue(8)：968-971,4.DOI:10.13699/j.cnki.1001-6821.2018.08.015
隐丹参酮对人乳腺癌MCF-7细胞周期调控因子表达的影响
Effect of cryptotanshinone on the expression of cell cycle regulatory factors in human breast cancer MCF-7 cells
摘要
Abstract
Objective To investigate effect of cryptotanshinone (CTS) on the proliferation and cell cycle of human breast cancer MCF-7 cells,and explore the mechanism.Methods The MCF-7cells were divided into 8 groups:blank group,control group,and experimental-Ⅰ,-Ⅱ,-Ⅲ,-Ⅳ,-Ⅴ,-Ⅵ groups.Blank group was given a blank medium solution;control group was given epirubicin at dose of 5.4 μmol · L-1;experimental-Ⅰ,-Ⅱ,-Ⅲ,-Ⅳ,-Ⅴ,-Ⅵ groups were given cultured medium solution containing CTS 1.5,3,6,12,24,48 μmol · L-1,respectively.After 24,48,72 h,the proliferation of cells was measured by CCK-8 (cell counting Kit-8).The distribution of MCF-7 cell cycle phase was detected by flow cytometry with PI single staining methods.The related protein expression of cell cycle regulation was detected by Western blotting.Results The results showed that CTS strongly inhibited the proliferation of MCF-7 cells,which were time and dose-dependent.The cell survival rates of blank group and the experimental-Ⅳ,-Ⅴ,-Ⅵ groups after 24 h were (100.12 ± 1.15) ％,(62.59 ± 2.16) ％,(48.43 ± 2.24) ％,(45.96 ± 1.36) ％,respectively;after 48 h,cell survival rates in the 4 groups were (100.24 ± 2.18)％,(50.41 ± 1.87)％,(41.32 ± 1.06)％,(37.74±1.14)％,respectively;after 72 h,cell survival rates in the 4 groups were (100.18 ± 2.46)％,(42.06 ± 1.13) ％,(38.62 ± 1.08) ％,(32.18 ± 1.35) ％,respectively.CTS groups had statistically significant differences compared with the blank group (all P ＜0.01).CTS affected the phase distribution of MCF-7 cell cycle,caused G2/M phase arrest,and induced the early apoptosis.The expression of Cyclin B1 and CDK1 proteins were down-regulated,and the expression of p53,p21cip1 and p27kip1 proteins were up-regulated significantly by CTS.Conclusion CTS had a significant value-added inhibition and regulated the cell cycle phase distribution on the MCF-7 cells,and its mechanism may be related to the up-regulation of p53 protein,the down-regulation of Cyclin B1 protein,inhibition of the kinase activity of CDK1.关键词
乳腺癌/MCF-7细胞/隐丹参酮/细胞周期/p53蛋白Key words
human breast cancer/MCF-7 cell/cryptotanshinone/cell cycle/p53 protein分类
医药卫生引用本文复制引用
叶因涛,钱钧强,杨惠莉,陈雷,胡利民..隐丹参酮对人乳腺癌MCF-7细胞周期调控因子表达的影响[J].中国临床药理学杂志,2018,34(8):968-971,4.基金项目
天津市科委创新平台建设基金资助项目(16PTSYJC00120) （16PTSYJC00120）
天津市卫计委中医中西医结合科研课题基金资助项目(2015066) （2015066）
