摘要
Abstract
Objective To observe the effect of glucosidorum tripterygii tororum on the expression of inflammatory factor and p38 mitogen-activated protein kinases (p38MAPK),nuclear factor-κB (NF-κB) p65 protein in ulcerative colitis rats.Methods Eight Wistar rats were randomly selected as normal group,without any treatment,24 rats were modeled on ulcerative colitis rats,2 rats dead,the remaining 22 rats were randomly divided into model group (n =7),test group (n =8) and positive control group (n =7).The normal group and model group were gavaged with 0.9％ NaCl 10 mL · kg-1,test group was gavaged with glucosidorum tripterygii tororum 20 mg · kg-1,positive control group was gavaged with sulfasalazine tablets 0.5 mg · kg-1,all rats were continuously gavaged for 14 d.The levels of serum inflammatory cytokines were measured by enzyme-linked immunosorbent assay,the histopathological changes of colon were observed by hematoxylin-eosin staining,the expression of p38MAPK and NF-κBp65 protein were detected by Western blotting.Results The serum IL-4 level in model group was significantly lower than that in normal group,IL-4 levels in test and positive control group were higher than those in model group (P ＜0.01).The level of serum IL-6 in model group was higher than those in normal group,test group and positive control group (P ＜ 0.01).The levels of serum IL-1β in model group,test group and positive control group were higher than that in normal group (P ＜ 0.01),the levels of IL-1β in test group and positive control group were lower than that in model group (P ＜ 0.01).The colon tissue injury score of model group,test group and positive control group were 4.56 ±0.88,1.65 ±0.53,1.50 ± 0.54,higher than that in normal group,which was 0.23 ± 0.32 (P ＜ 0.01),compared with model group,the scores of colonic tissue injury in test group and positive control group decreased (P ＜0.01).The relative expression of p38MAPK and NF-κBp65 in model group were 1.62 ± 2.39,1.53 ± 3.21,higher than those in normal group,wihch were 0.74 ±0.11,0.63 ±0.09.The relative expression levels of p38MAPK protein in test group and positive control group were 0.93 ± 0.16,0.78 ± 0.19,the relative expression levels of NF-κBp65 protein were 0.81 ± 0.23,0.72 ± 0.16,lower than model group (all P ＜ 0.01).Conclusion Glucosidorum tripterygii tororum can effectively reduce the levels of serum inflammatory cytokines in ulcerative colitis rats and improve the morphology of colonic mucosa.The molecular substance may be related to the decrease of p38MAPK and NF-κBp65 protein expression and the regulation of inflammatory pathway.关键词
雷公藤多苷/溃疡性结肠炎/炎症因子/p38活化蛋白激酶/核因子-κBp65Key words
glucosidorum tripterygii tororum/ulcerative colitis/inflammatory factor/p38 mitogen-activated protein kinase/nuclear factor-κBp65分类
医药卫生
