中国美容医学2018，Vol.27Issue(4)：109-111,3.
miRNA-769-5p上调对正常人成纤维细胞生物活性的影响
Effect of miRNA-769-5p on Biological Activity of Normal Human Fibroblasts
倪娜 1周炳荣 1马委委 1骆丹1
作者信息
- 1. 南京医科大学第一附属医院皮肤科 江苏 南京 210029
- 折叠
摘要
Abstract
Objective To investigate the effect of miRNA-769-5p on biological activity in normal human fibroblasts. Methods Human fibroblasts were transfected with miRNA-769-5p mimic. CCK-8 kit was performed to test proliferation rate. Cell were observed under fluorescence microscope. Flow cytometer was used to measured rate of reactive oxygen species and apoptosis. All the parameters of the mimics group were compared with the control group. Results The relative expression of miRNA-769-5p in the mimics group was (1.36±0.2), which was significantly higher than that of the control group (1.0±0.1), the difference was statistically significant(P<0.05). The cell proliferation rate in the mimics group (0.747±0.072) was significantly lower than that of the control group (1.007±0.025)(P<0.05). The fluorescence value of the mimics group (92.067±3.092) was significantly higher than that of the control group (21.600±8.487)(P<0.05). The apoptotic rate in the mimics group was (49.299 ± 5.184)%, which was significantly higher than that of the control group (5.977±0.939)%(P<0.05). Conclusion Up-regulation of miRNA-769-5p induce photo-damage and have effect on biological activity in normal human fibroblasts.关键词
miRNA/成纤维细胞/紫外线/光损伤Key words
miRNA/fibroblasts/ultraviolet/photodamage分类
医药卫生引用本文复制引用
倪娜,周炳荣,马委委,骆丹..miRNA-769-5p上调对正常人成纤维细胞生物活性的影响[J].中国美容医学,2018,27(4):109-111,3.
