中国水产科学2018，Vol.25Issue(2)：269-277,9.DOI:10.3724/SP.J.1118.2018.17177
河南境内四水系宽鳍鱲野生群体的遗传多样性
Genetic diversity of wild Zacco platypus populations among four river systems in Henan Province analyzed by mitochondrial CO I gene
摘要
Abstract
To correctly determine the genetic structure of wild Zacco platypus populations from Haihe, Yellow, Huaihe, and Yangtze River basins in Henan Province, we analyzed the population genetic diversity and differen-tiation of the species using mitochondrial CO I gene. A total of 110 individuals were sequenced, which have de-fined 40 distinct haplotypes. The average haplotype diversity and nucleotide diversity were 0.92077 and 0.02439, respectively. Analysis of molecular variance (AMOVA) indicated that most of the genetic variation existing among populations (60.59%), and the remaining 37.67% of genetic variation was attributed to differentiation within populations. The assignment of populations based on Neighbor-Joining (NJ) method revealed that Z. platypus populations from Yangtze River divided into two distinct mitochondrial DNA lineages, one clustered from the ZY population and the other clustered individuals from Haihe River, Yellow River and Huaihe River. This phyloge-netic pattern is not consistent with the previous reported North and South Clades. Demographical history analysis indicated that Z. platypus populations had undergone a bottleneck which coincided with the middle Pleistocene and suffered from climate changes. Considering the genetic and phylogeographic results obtained in this study, appropriate measures are proposed to conserve Z. platypus population, especially for those with low genetic di-versity in Haihe River basin.关键词
宽鳍鱲/CO I/遗传多样性/群体遗传结构Key words
Zacco platypus/CO I/genetic diversity/population genetic structure分类
农业科技引用本文复制引用
刘慧芬,孔祥会,聂国兴,张超,王静,顾钱洪,周传江,孟晓林,张建新,宋东蓥,李学军..河南境内四水系宽鳍鱲野生群体的遗传多样性[J].中国水产科学,2018,25(2):269-277,9.基金项目
河南省科技攻关重点项目(142102110057,162102310443,162102110147,172102310751) （142102110057,162102310443,162102110147,172102310751）
河南省高校科技创新团队支持计划(14IRTSTHN013) （14IRTSTHN013）
河南省创新型科技团队支持计划(CXTD2016043). （CXTD2016043）
