中国水产科学2018，Vol.25Issue(2)：278-285,8.DOI:10.3724/SP.J.1118.2018.17202
齐口裂腹鱼SNP标记与生长性状的关联分析
Association analysis between SNP markers and growth-related traits in Schizothorax prenanti
摘要
Abstract
Schizothorax prenanti,an economically important,cold-water fish species,has rich nutritional value and high economic value. With the increase in artificial breeding and breeding intensification, the germplasm re-sources of S.prenanti have been degraded,which is revealed in individual miniaturization,slow growth,and de-creased disease resistance. Therefore, it is necessary to screen the molecular markers of growth traits and use mo-lecular-marker-assisted breeding. In order to acquire some reliable molecular genetic markers for growth-related traits,the correlation analysis of 26 SNP markers and growth-related traits in S.prenanti were analyzed using 114 samples with the same growth conditions. A principal component analysis showed that body weight accounted for 93.42% of the variance, the eigenvalue was greater than 1 and the accumulative variance ratio was more than 85%, and it was the first principal component of the growth traits of S.prenanti.Correlation analysis between genotypes of SNPs and growth traits indicated that ug17711-0-2201 was significantly correlated with total length (P<0.05), and ug24013-0-3669 showed a significant influence on total length and body length (P<0.05). There was a sig-nificant association between ug25050-0-1678 and body weight, body height, total length, and body length (P<0.05); ug22712-0-2452 and the body weight, body height, and body length were significantly associated (P<0.01). We also estimated that the genetic diversity parameters for 4 loci were significantly correlated with the growth traits. The mean observed heterozygosity, expected heterozygosity, and polymorphism information content (PIC) were 0.2369, 0.2110 and 0.17 respectively. The polymorphism of ug25050-0-1678 was moderate (0.25≤PIC<0.5). In conclusion, ug25050-0-1678 and ug22712-0-2452 were significantly associated with growth traits, and could be used as important candidate molecular markers for breeding selection of S. prenanti. Our results could provide an effective basis for the study of genetic improvement and selective breeding in S.prenanti.关键词
齐口裂腹鱼/SNP标记/生长性状/关联分析Key words
Schizothorax prenanti/SNP markers/growth traits/correlation analysis分类
农业科技引用本文复制引用
杨月静,向梦斌,叶祥益,张争世,罗辉,叶华..齐口裂腹鱼SNP标记与生长性状的关联分析[J].中国水产科学,2018,25(2):278-285,8.基金项目
国家自然科学基金资助项目(31402302) （31402302）
中央高校基本科研业务费资助项目(XDJK2017B008) （XDJK2017B008）
西南大学荣昌校区青年基金资助项目(20700938). （20700938）
