中国水产科学2018，Vol.25Issue(2)：325-335,11.DOI:10.3724/SP.J.1118.2018.17190
中华绒螯蟹微卫星多重PCR体系的建立及其亲子鉴定应用
Development and application of multiplex PCR panels of microsatel-lites in Chinese mitten crab(Eriocheir sinensis)
摘要
Abstract
In order to increase the analysis efficiency of microsatellites in Chinese mitten crab(Eriocheir sinensis), we report four highly informative, multiplex PCR panels developed from 16 microsatellite markers. The multiplex PCR panels were used to evaluate the genetic diversities and parentage assignment for 95 individuals in 3 families. The results showed that the 16 microsatellite markers screened in this study were rich in polymorphism, for ex-ample the average of observed heterozygosity (Ho) was 0.8206, the average of expected heterozygosity (He) was 0.8164, and the average of polymorphism information content (PIC) was 0.7927. Cervus 3.0 software was used to determine parentage assignment for 95 individuals in 3 families, and the results revealed that the accuracy of par-entage assignment using any 2 groups of multiplex PCR panels was more than 94.74%. When using any 3 groups of multiplex PCR panels, the accuracy of parentage assignment was greater than 98.95%. The accuracy of parentage assignment was greater than 100% using all four multiplex assays. Using the combination of set 1, set 2, and set 3, or the combination of set 1, set 3, and set 4, the accuracy of parentage assignment also reached 100%, so the selection of these combinations of multiplex PCR panels can not only get accurate genealogy information, but also reduce workload and costs. Therefore, these multiplex PCR panels can provide a convenient and efficient way for breeding and family management of E.sinensi.关键词
中华绒螯蟹/微卫星/多重PCR/亲子鉴定Key words
Eriocheir sinensis/microsatellites/multiplex PCR/parentage assignment分类
农业科技引用本文复制引用
肖起珍,刘青,李清清,郑海地,吴旭干,成永旭..中华绒螯蟹微卫星多重PCR体系的建立及其亲子鉴定应用[J].中国水产科学,2018,25(2):325-335,11.基金项目
农业部现代农业产业技术体系项目[农科(产业)函[2017]第23号] （产业）
上海市科技兴农推广项目[沪农科推字(2015)第1-7号] （2015）
上海市科委工程技术中心能力提升项目(16DZ2281200) （16DZ2281200）
上海高校水产学高峰学科建设项目(2015-62-0908) （2015-62-0908）
深圳市澳华农牧有限公司横向项目(D-8006-15-0054). （D-8006-15-0054）
