中国水产科学2018，Vol.25Issue(2)：361-372,12.DOI:10.3724/SP.J.1118.2018.17180
玉米蛋白粉对大黄鱼生长、肠道组织结构及肠道菌群的影响
Effects of replacing fish meal with corn gluten meal on growth, intes-tinal histology, and intestinal microbiota of large yellow croaker, Larimichthys crocea
摘要
Abstract
This study investigated the effects of the partial substitution of dietary fish meal by corn-gluten meal on the growth,intestinal histology,and structure of intestinal bacteria in juvenile large yellow croaker(Larimichthys crocea) (mean initial weight 10.49 g±0.03 g). Six isonitrogenous (45% crude protein) and isolipidic (10% crude lipid) diets with various dietary protein levels (0%, 15%, 30%, 45%, 60%, and 75%, designated as FM, CGM15, CGM30, CGM45, CGM60, and CGM75, respectively) were formulated and fed to triplicate groups for 56 d. The results showed that the survival rate (SR), specific growth rate (SGR), and feed conversion ratio (FCR) were not significantly affected (P>0.05). The observed intestinal histology (mucosa thickness, mucosal fold height, and goblet cell quantity) was not significantly affected in all of the groups (P>0.05), but some epithelial cells in the CGM60 group were destroyed or rearranged in a disorderly manner and parts of the striated border and intestinal villus were destroyed, the mucosal fold height in the CGM75 group was lower than that in the other groups, and the density and numbers of mucosal folds decreased. An Illumina MiSeq high-throughput sequencing analysis of intestinal contents showed that the Chao1, Shannon, and Simpson indices, and Good's coverage in FM, CGM15, and CGM75 were not significantly affected (P>0.05). The Firmicutes and Proteobacteria groups completely dominated the bacterial communities in juvenile L.crocea.The Paenibacillus,Alkaliphilus,Bacillus,and Entero-coccus genera were the most dominant bacteria in the microbial ecosystem. At the genus level, compared to the control group, the abundances of Streptococcus in CGM15 and CGM75 were significantly higher (P<0.05), the abundances of Devosia and Cellvibrio in CGM15 were significantly lower (P<0.05), and the abundance of Staphylococcus in CGM75 was significantly higher (P<0.05). These results suggest that the optimal die-tary-protein level is about 45% without adverse effects on growth, intestinal histology, and structure of intestinal dominant bacteria in juvenile L.crocea.关键词
大黄鱼/玉米蛋白粉/生长/肠道组织结构/高通量测序/肠道细菌Key words
Larimichthys crocea/corn gluten meal/growth/intestinal histology/high-throughput sequencing/in-testinal microbiota分类
农业科技引用本文复制引用
何娇娇,王萍,冯建,娄宇栋..玉米蛋白粉对大黄鱼生长、肠道组织结构及肠道菌群的影响[J].中国水产科学,2018,25(2):361-372,12.基金项目
国家自然科学基金项目(31602205) （31602205）
国家海洋公益性行业科研专项(201505025) （201505025）
浙江省自然科学重点基金(Z16E0-90006) （Z16E0-90006）
浙江省重中之重学科开放基金(XKZSC1406) （XKZSC1406）
舟山市海洋专项(2015C41001) （2015C41001）
浙江海洋大学重中之重学科项目(20161105). （20161105）
