中国现代医学杂志2018，Vol.28Issue(14)：35-39,5.DOI:10.3969/j.issn.1005-8982.2018.014.007
TIM-1和TIM-4 mRNA在HAV患者中的表达及其临床意义
Clinical significance of TIM-1 and TIM-4 mRNA levels in patients with hepatitis A
摘要
Abstract
Objective To observe the expression levels of T cell immunoglobulin domain and mucin domain 1 (TIM-1) and TIM-4 mRNAs in peripheral blood of the patients with typical courses of viral hepatitis A, and to investigate the relationships of TIM-1 and TIM-4 mRNAs with RNA content of HAV, concentrations of anti-HAV IgM and IgG, and parameters of hepatic function. Methods Blood specimens were taken from 30 patients with typical courses of viral hepatitis A in incubation or pre-icterus stage, icterus stage and recovery stage and 30 healthy controls. TIM-1 and TIM-4 mRNA levels in the peripheral blood were assayed by RT-PCR. Also the HAV RNA content in the peripheral blood was detected. The concentrations of HAV antibody IgM and IgG in the peripheral blood of the viral hepatitis A patients were assayed by ELISA. The parameters of hepatic function (ALT, T-bil and D-bil) were assayed by the Hitachi 7600 Auto-biochemical Analyzer. Results The expression levels of TIM-1 and TIM-4 mRNAs in the peripheral blood of the hepatitis A patients were dramatically higher than those of the control group (P < 0.05), reached the peak value at the icterus stage and declined during the recovery stage. The expression levels of TIM-1 and TIM-4 mRNAs were in positive correlations with the titer of anti-HAV IgM and the parameters of hepatic function in the hepatitis A patients (P < 0.05), but did not have correlation with the titer of anti-HAV IgG (P > 0.05). Conclusions Expression levels of TIM-1 and TIM-4 mRNAs in the peripheral blood of hepatitis A patients have high degree to match the development of hepatitis A. TIM-1 and TIM-4 mRNAs are likely related to the incidence and development of hepatitis A.关键词
甲型病毒性肝炎/T细胞免疫球蛋白域蛋白-1/T细胞免疫球蛋白域蛋白-4Key words
viral hepatitis A/TIM-1/TIM-4分类
医药卫生引用本文复制引用
胡耀华,阳慧,吴翔,符春苗,邵运禄,郑才玲,卓书伟..TIM-1和TIM-4 mRNA在HAV患者中的表达及其临床意义[J].中国现代医学杂志,2018,28(14):35-39,5.基金项目
海南省卫生厅课题(No:15A200012) （No:15A200012）
