中国药房2018，Vol.29Issue(9)：1189-1193,5.DOI:10.6039/j.issn.1001-0408.2018.09.09
黄芪甲苷对D-半乳糖诱导原代培养心肌细胞凋亡的保护作用及机制研究
Study on Protective Effect and Mechanism of Astragaloside Ⅳ on D-galactose-induced Primary Cardiomyocytes Apoptosis
摘要
Abstract
OBJECTIVE:To study the protective effect and mechanism of astragaloside Ⅳ on D-galactose(D-gal)-induced primary cardiomyocytes apoptosis. METHODS:Wistar neonatal rat primary cardiomyocytes were isolated and cultured. The cardiomyocytes were divided into normal control group(DMEM high glucose medium without serum),D-gal group(DMEM high glucose medium without serum containing 5 g/L D-gal)and astragaloside Ⅳ low-concentration,medium-concentration and high-concentration groups(after cultured with DMEM high glucose medium without serum containing 25,50,100 μg/mL astragaloside Ⅳ for 1 h,and then replaced with DMEM high glucose medium without serum containing corresponding concentration of astragaloside Ⅳ and 5 g/L D-gal). Cardiomyocytes activity was detected by CCK-8 kit after cultured for 48 h.The apoptosis level was detected by Hoechst staining(cultured for 24 h)and flow cytometry(cultured for 48 h). The mRNA expressions of apoptosis related factors(Bcl-2,Bax,Caspase-3)and ANP in cardiomyocytes were detected by RT-PCR after cultured for 24 h. RESULTS:Compared with normal control group,the activity of cardiomyocytes,mRNA level of Bcl-2 and ratio of Bcl-2/Bax were decreased in D-gal group,while apoptosis rate and mRNA levels of Bax,Caspase-3 and ANP were increased, with statistical significance(P<0.05 or P<0.01). Compared with D-gal group,the activity of cardiomyocytes,mRNA level of Bcl-2 and ratio of Bcl-2/Bax were increased in astragaloside Ⅳ groups,while apoptosis rate and mRNA levels of Bax,Caspase-3 and ANP were decreased,with statistical significance(P<0.05 or P<0.01),and in concentration-dependent manner. CONCLUSIONS:Astragaloside Ⅳ can protect D-gal induced primary cardiomyocytes from apoptosis,the mechanism of which may be associated with the increase of Bcl-2/Bax ratio and the decrease of mRNA expressions of Caspase-3 and ANP.关键词
黄芪甲苷/D-半乳糖/原代心肌细胞/凋亡/保护作用Key words
AstragalosideⅣ/D-galactose/Primary cardiomyocytes/Apoptosis/Protective effect分类
医药卫生引用本文复制引用
王晓霞,刘天龙,刘晶,刘小玲,张勇,肖云峰,刘小雷..黄芪甲苷对D-半乳糖诱导原代培养心肌细胞凋亡的保护作用及机制研究[J].中国药房,2018,29(9):1189-1193,5.基金项目
国家自然科学基金资助项目(No.81460066) （No.81460066）
