中国药房2018，Vol.29Issue(9)：1213-1217,5.DOI:10.6039/j.issn.1001-0408.2018.09.15
LC-MS/MS法同时测定回春育子颗粒中9种成分的含量
Simultaneous Determination of 9 Components in Huichun yuzi Granules by LC-MS/MS
摘要
Abstract
OBJECTIVE:To establish the method for simultaneous determination of 9 components in Huichun yuzi granules. METHODS:LC-MS/MS method was adopted. The determination was performed on Shim-pack XR-ODS C18column with column temperature of 30 ℃. The sample size was 5 μL,and ion source as electrospray ion source;MRM mode was adopted. The acetonitrile-water was used as mobile phase for ferulic acid,rutin,paeonol,icariin and schisandrin(gradient elution);positive ion mode monitoring was conducted. The methanol-0.1% fomic acid water was used as mobile phase for naringin,verbascoside, amygdalin and protocatechuic acid(gradient elution);negative ion mode monitoring was conducted. RESULTS:The linear ranges of ferulic acid,rutin,paeonol,icariin,schisandrin,naringin,verbascoside,amygdalin and protocatechuic acid were 0.499 5-500, 25-1 000,0.245 9-250,5.185 1-1 000,0.981 9-1 000,0.248 1-125,2.510 4-250,10-2 500,4.999 7-1 000 ng/mL(r≥0.997 8), respectively. The limits of detection were 0.075,0.30,0.072,0.015,0.015,0.075,0.15,0.30,0.15 ng/mL,respectively;the limits of quantitation were 0.25,1.00,0.24,0.51,0.49,0.25,0.50,0.99,0.49 ng/mL,respectively. The recoveries rate were 91.39%-103.56%(RSD=1.03%-3.67%,n=6).RSDs of stability test ranged 1.4%-3.4%(n=6)within 48 h at room temperature. CONCLUSIONS:The method is rapid,sensitive and reproducible,and can be used for content determination of 9 components in Huichun yuzi granules.It can be used for quality control of Huichun yuzi granules.关键词
回春育子颗粒/成分/液相色谱-串联质谱法/质量控制/含量测定Key words
Huichun yuzi granules/Component/LC-MS/MS/Quality control/Determination of content分类
医药卫生引用本文复制引用
薛刚强,曹宝帅,高淑丽,张健,潘新艳..LC-MS/MS法同时测定回春育子颗粒中9种成分的含量[J].中国药房,2018,29(9):1213-1217,5.基金项目
河北省医学科学研究课题计划项目(No.20130293) （No.20130293）
