中国药房2018，Vol.29Issue(9)：1218-1221,4.DOI:10.6039/j.issn.1001-0408.2018.09.16
UPLC-Q-TOF/MS法分析鉴定对叶百部碱在大鼠体内的代谢产物
Identification and Analysis of Tuberostemonine Metabolites in Rats by UPLC-Q-TOF/MS
摘要
Abstract
OBJECTIVE:To analyze and identify the metabolites of tuberostemonine in rats plasma,urine and feces,and to clarify metabolic pathway of tuberostemonine. METHODS:Rats were randomly divided into blank group(0.3% carboxymethyl cellulose)and medication group(tuberostemonine 50 mg/kg,i.g.),with 6 rats in each group.The plasma of rats was collected 0.25, 0.5,1,2,4,6,12 h after intragastric administration to prepare samples. Urine and feces were collected 0-12 h and 12-24 h after administration to prepare samples. UPLC-Q-TOF/MS and software of Triple TOFTMhigh resolution mass spectrometry were used to analyze and identifiy chemical structure of metabolites in samples. RESULTS:A total of 4 compounds were detected in rat plasma, including one prototype compound and 3 metabolites. 4 metabolites were detected in urine and 2 metabolites in feces. Phase Ⅰmetabolites of tuberostemonine mainly included hydration and hydroxylation. Phase Ⅱ metabolites were not found. CONCLUSIONS:Hydration and hydroxylation are the major metabolic transformation forms of tuberostemonine in rats in vivo.关键词
对叶百部碱/超高效液相色谱-四级杆-飞行时间质谱法/代谢产物/鉴定Key words
Tuberostemonine/UPLC-Q-TOF/MS/Metabolite/Identification分类
医药卫生引用本文复制引用
董巍,巫秋萍,梁鑫,崔涛,潘虹..UPLC-Q-TOF/MS法分析鉴定对叶百部碱在大鼠体内的代谢产物[J].中国药房,2018,29(9):1218-1221,4.基金项目
齐齐哈尔市科学技术计划项目(No.SFGG-201544) （No.SFGG-201544）
