中国医学科学院学报2018，Vol.40Issue(2)：225-232,8.DOI:10.3881/j.issn.1000-503X.2018.02.013
急慢性特发性血小板减少性紫癜患儿的差异表达基因特征及交互作用网络分析
Analysis of Differentially Expressed Genes and the Interaction Network between Acute and Chronic Idiopathic Thrombocytopenic Purpura in Children
摘要
Abstract
Objective To analyze the differentially expressed genes and key proteins in T cells between acute and chronic idiopathic thrombocytopenic purpura (ITP) in children and provide the basis for the prevention and therapies of this disease.Methods Microarray gene chip data from T cells of children with acute or chronic ITP were downloaded from the GEO Database.The gene expression profiles,gene function,and protein interaction network were analyzed by R,QOE,Networkanalyst,GCBI,and GenClip.Results The gene expression profiles between these two groups were significantly different.Among the 54 675 genes analyzed,there were 457 (0.84％) differentially expressed genes between these two groups.In the protein interaction networks among top 20 differentially expressed genes,the core was JUN (down-regulated) and ITCH (up-regulated),which were both related to the tumor necrosis factor signaling pathway;differentially expressed genes were mainly related to the activation and tolerance of T cell.Conclusions The gene expression profiles differ between acute and chronic ITP patients,suggesting that the gene transcription profile plays a regulatory role in the different stages of ITP.JUN and ITCH may play a role in predict the progression of ITP and may exert their biological functions by regulating the tumor necrosis factor signaling pathway.关键词
特发性血小板减少性紫癜/差异表达基因/转录谱/生物信息学Key words
idiopathic thrombocytopenic purpura/differentially expressed genes/transcriptional spectra/bioinformatics分类
医药卫生引用本文复制引用
任晓梅,刘启玲,辛宝,张荣强..急慢性特发性血小板减少性紫癜患儿的差异表达基因特征及交互作用网络分析[J].中国医学科学院学报,2018,40(2):225-232,8.基金项目
陕西省科技厅自然科学基础研究计划项目(2016JM8031)Supported by the Natural Science Foundation of Shaanxi Provincial Department of Science and Technology (2016JM8031) （2016JM8031）
