广东海洋大学学报2018，Vol.38Issue(2)：7-14,8.DOI:10.3969/j.issn.1673-9159.2018.02.002
金钱鱼IGF-1和IGF-2的克隆及其在胚胎发育过程的表达
Insulin-like Growth Factors 1 and 2 in Spotted Scat(Scatophagus argus):Molecular Cloning and Differential Expression During Embryonic Development
摘要
Abstract
[Objectives]Insulin-like growth factors -1and-2 (IGF-1and 2) are critical factors involved in the regulation of embryonic development in vertebrates.[Method]In this study, temporal expression patterns throughout embryonic development were examined by quantitative real-time PCR assay.[Result] Two IGF cDNAs, i.e. IGF-1 and IGF-2 mRNAs, were detected in embryonic stages of spotted scat by Real-Time PCR.The IGF-1 and IGF-2 cDNA sequences encoded proteins of 186 and 215 amino acid residues, respectively. IGF1 and IGF2 are sequentially composed of a signal peptide, B, C, A, D ,E domains and six conservative cysteine residues. The deduced amino acid sequence of C. semilaevis IGF-I gene has highest identity with those of other perciformes fishes. IGF-1 mRNA level remained high from fertilized eggs to blastula.In contrast,IGF-2 mRNA level was low from fertilized eggs to blastula and peaked at gastrula and neurula followed by a gradual decrease. [Conclusion]The spotted scat IGF-1 amino acid sequences shared the lower sequence identity with IGF-2, however, both of them have a higher homology with Perciformes, and play differential roles in the embryonic development of spotted scat.关键词
金钱鱼/IGF-1/IGF-2/基因克隆/胚胎发育Key words
Scatophagus argus/IGF-1/IGF-2/gene clone/embryonic development分类
生物科学引用本文复制引用
张克伟,陈华谱,江东能,邓思平,朱春华,吴天利,李广丽..金钱鱼IGF-1和IGF-2的克隆及其在胚胎发育过程的表达[J].广东海洋大学学报,2018,38(2):7-14,8.基金项目
湛江市财政资金科技竞争性分配项目(2016A03017,2017A03019) （2016A03017,2017A03019）
广东省自然科学基金(2016A030313743) （2016A030313743）
广东高校省级重点平台和重大科研项目(2015KTSCX058) （2015KTSCX058）
