广东海洋大学学报2018，Vol.38Issue(2)：15-22,8.DOI:10.3969/j.issn.1673-9159.2018.02.003
企鹅珍珠贝Sox9基因的克隆及表达分析
Molecular Cloning and Expression Analysis of Sox9 Gene from Pteria penguin
摘要
Abstract
[Objective]To better understand gender conversion mechanism of Petria penguin.[Method]Full-length cDNA of Sox9 gene from Pteria penguin was cloned by RACE-PCR and its physicochemical properties and evolutionary status were analyzed. Fluorescent quantitative PCR was used to analyze the expression of Sox9 gene indifferent tissues and gonads at different life cycle stages.[Result and conclusion]The full-length cDNA of Sox9 gene was 2 267 bp, with the longest open reading frame (ORF) of 1 410 bp, It encoded for a protein of 469 amino acids. Amino acid sequence alignment showed that the Sox9 gene in Pteria penguin shared highly sequence identity with Sox9 gene of Pinctada margaritifera and Pinctada fucata(>81%).The realtime-PCR results showed that Sox9 was expressed in all tissues. The expression level was highest in foot (P < 0.05), followed by testis. The expression analysis in gonads of different periods indicated that Sox9 had the highest expression in mature testes, but lower expression in early testis, emission testis and mature ovary, with the lowest level in early ovary.关键词
企鹅珍珠贝/Sox9/PCR/基因克隆Key words
Pteria penguin/Sox9/PCR/gene clone分类
生物科学引用本文复制引用
许开航,王梅芳,余祥勇,于非非,林丹丹,郑煜东,李启辉,万绮娟,陈荣娴..企鹅珍珠贝Sox9基因的克隆及表达分析[J].广东海洋大学学报,2018,38(2):15-22,8.基金项目
广东省科技发展专项(2016A020210115) （2016A020210115）
广东省渔港建设和渔业发展专项(B201601-Z08,Z2014005) （B201601-Z08,Z2014005）
广东海洋大学博士科研启动项目(E15041) （E15041）
广东海洋大学创新强校重点课题(GDOU2016050248) （GDOU2016050248）
