浙江医学2018，Vol.40Issue(5)：433-438,6.DOI:10.12056/j.issn.1006-2785.2018.40.5.2017-2588
IRF3调控瘢痕疙瘩成纤维细胞增殖和细胞外基质表达的研究
IRF3 regulates proliferation of fibroblasts and expression of extracellular matrix proteins in keloid
摘要
Abstract
Objective To investigate the expression of Interferon regulatory factor 3 (IRF3) in keloid,and its function in regulating proliferation of keloid-derived fibroblasts(KFs) and expression of extracellular matrix(ECM) proteins.Methods Ten tissue samples of keloid and 8 samples of normal skin were cultured and fibroblasts were isolated.The expression of IRF3 mRNA and protein in human keloid tissues were detected by qRT-PCR and Western blotting,respectively.KFs were incubated with siRNAs-IRF3 or scramble for 48 h;then cells were treated with TGF-β1 (10 ng/ml) for 24 h.KFs proliferation,the expression of type Ⅰ collagen,a-SMA,TGF-β RI/Ⅱ,p-Smad2,Smad2,p-Smad3 and Smad3 proteins were detected.Results Expression of IRF3 mRNA and proterin was significantly higher in human keloid tissues than that in normal skin tissues.Down-regulation of IRF3 significantly inhibited KF proliferation and the expression of type Ⅰ collagen,a-SMA,TGF-β receptor Ⅰ and Ⅱ in TGF-β1-stimulated KFs.Furthermore,down-regulation of IRF3 suppressed the phosphorylation levels of Smad2 and Smad3 in human KFs induced by TGF-β1.Conclusion Down-regulation of IRF3 inhibits the proliferation and ECM expression in KFs via suppressing the TGF-β1/Smad signaling pathway.IRF3 may represent a novel target for treatment of the keloid disease.关键词
干扰素调节因子3/瘢痕疙瘩成纤维细胞/细胞外基质/TGF-β1/Smad通路Key words
IRF3/KFs/ECM/TGF-β1/Smad pathway引用本文复制引用
张谊,洪炜龙,徐云升,李智铭,张璃,林孝华,张启瑜..IRF3调控瘢痕疙瘩成纤维细胞增殖和细胞外基质表达的研究[J].浙江医学,2018,40(5):433-438,6.基金项目
浙江省医药卫生一般研究计划项目(2015KYB244) （2015KYB244）
浙江省教育厅高教重中之重学科(2008-255) （2008-255）
