摘要
Abstract
Objective To investigate the effect of LncRNA NNT-AS1 on proliferation and apoptosis of gastric carcinoma cells and its mechanism.Methods The expression levels of LncRNA NNT-AS1 in the gastric carcinoma cell lines KATO-Ⅲ,NUGC-3,AGS,N87 and normal gastric cell line NSFC was determined by qRt-PCR.The KATO-Ⅲ cells were transfected with negative shRNA-Lenti (vector group) or Lenti-sh-NNT-AS1 (sh-NNT-AS1 group).The cell proliferation ability was measured by MTT assay,apoptosis was tested by flow cytometry,the expression of Caspase 3,8,9 protein was measured by Western blot.Results The relative express levels of LncRNA NNT-AS1 in KATO-Ⅲ,NUGC-3,AGS and N87 cells were significantly higher than that in normal gastric NSFC cells (P＜0.05).Mlr assay demonstrated that the OD490 value of sh-NNT-AS1 group was significantly lower than control and vector group after culturing for 12,24,48 and 72 h (P＜0.05).The number of clone formation cells in sh-NNT-AS1 group was significantly lower than that in control group and vector group (P＜0.01).The apoptosis rate of sh-NNT-AS1 group was significantly higher than that in vector and control group (P＜0.01).The expression level of cleaved Caspase 3,9 protein in sh-NNT-AS1 group was significantly higher than that in vector group (P＜0.05,P＜0.01);while,there was no significantly difference in expression of cleaved caspase-8 protein between sh-NNT-AS1 and vector groups (P ＞0.05).Conclusion LncRNA NNT-AS1 may promote proliferation and inhibit apoptosis of gastric carcinoma cells,which may is associated with up-regulation of Caspase 3 and 9.关键词
LncRNA NNT-AS1/胃癌/增殖/凋亡Key words
LncRNA NNT-AS1/Gastric carcinoma/Proliferation/Apoptosis
