浙江医学2018，Vol.40Issue(6)：554-556,559,4.DOI:10.12056/j.issn.1006-2785.2018.40.6.2017-1140
RP-HPLC法测定急性溴苯腈中毒小鼠肝组织腺苷酸含量
Determination of adenosine content in liver tissue of mice with acute bromoxynil intoxication by RP-HPLC
摘要
Abstract
Objective To develop a new RP-HPLC method for determination of adenosine content and to evaluate the effect of bromoxynil on liver energy metabolism in mice.Methods A C18ODS column was used in RP-HPLC with acetonitrile phosphate buffer as mobile phase,the detection wavelength was 254 nm,the column temperature was 16℃ and the flow rate was 1.1 ml/min.Adenosine (ATP,ADP and AMP) contents in the liver tissue of mice were measured.Results The average recovery rates of ATP,ADP and AMP were (93.63 ± 8.02)％,(87.02 ± 8.28)％ and (94.99 ± 6.97)％,respectively.The relative standard deviations (RSDs) of within-day were 0.79％,1.02％ and 1.05％;and the RSDs of between-days were 2.09％,1.75％ and 2.17％,respectively.Conclusion The RP-HPLC method is simple,rapid and sensitive in analysis of the adenosine contents in mouse liver.Bromoxynil can cause hepatic injury and disturb liver energy metabolism in mice.关键词
反相高效液相色谱法/肝/腺苷酸/溴苯腈Key words
RP-HPLC/Liver/Adenosine/Bromoxynil引用本文复制引用
周小明,翁杰,王志翊,龚裕强..RP-HPLC法测定急性溴苯腈中毒小鼠肝组织腺苷酸含量[J].浙江医学,2018,40(6):554-556,559,4.基金项目
浙江省自然科学基金项目(LY15H150008) （LY15H150008）
浙江省卫生厅资助项目(2014KYB153) （2014KYB153）
