浙江医学2018，Vol.40Issue(7)：676-679,后插1,5.DOI:10.12056/j.issn.1006-2785.2018.40.7.2017-716
子宫平滑肌前列腺素E2受体分布与复发性自然流产发病机制的相关性研究
Relationship between the PGE2 receptor distribution in uterine smooth muscle and the pathogenesis of recurrent spontaneous abortion
摘要
Abstract
Objective To investigate the relationship between the distribution of prostaglandin E receptor (EP) in uterine smooth muscle cells and the pathogenesis of recurrent spontaneous abortion (RSA).Methods Thirty seven patients with RSA (RSA group) and 48 women with induced abortion (control group) were enrolled.Expression levels of cyclooxygenase-2 (COX-2) and membrane associated prostaglandin E synthase (mPGES) in decidual tissue,and EP in uterine smooth muscle cells were analyzed by RT-PCR.Prostaglandin E2 (PGE2) in uterine decidua tissue and cyclic adenosine monophosphate (cAMP) in uterine smooth muscle cells were detected by ELISA.Intracellular calcium ion was labeled with Fluo-3AM and images were acquired with Confocal microscope.Results The expressions of COX-2,mPGES and PGE2 in the decidua tissue of RSA group were all higher than those of control group (all P＜ 0.05).The expression levels of EP1 and EP3 in uterine smooth muscle cells of group RSA were all higher than those of control group,while EP2 and cAMP were all lower (all P＜ 0.05).The calcium ion concentration in the RSA group was significantly higher than that in the control group (P＜ 0.01).Conclusion The increase of PGE2 synthesis in the uterine decidua tissue and the abnormal distribution of EP receptor in uterine smooth muscle cells may induce abnormal contraction of the uterus and lead to RSA.关键词
复发性自然流产/子宫平滑肌细胞/前列腺素E2/前列腺素E2受体Key words
Recurrent spontaneous abortion/Smooth muscle/Prostaglandin E2/Prostaglandin E2 receptor引用本文复制引用
李旭升,洪丽霞,金庆跃..子宫平滑肌前列腺素E2受体分布与复发性自然流产发病机制的相关性研究[J].浙江医学,2018,40(7):676-679,后插1,5.基金项目
浙江省自然科学基金项目(LY12H04003) （LY12H04003）
