摘要
Abstract
Objective To assess the diagnostic value of human papilloma virus (HPV) E6/E7 mRNA test in triage of patients with atypical squamous cell of undetermined significance (ASC-US) after cervical conization.Methods A total of 160 patients after cervical conization with ASC-US cytology underwent colposcopic examination and cervical biopsy,and HPV E6/E7 mRNA and high-risk HPV DNA (HR-HPV DNA) tests were performed.With pathological results as the gold standard,the diagnostic value of HPV E6/E7 mRNA in patients with ASC-US after cervix conization was analyzed and compared with HR-HPV DNA.Results The overall positive rate of HPV E6/E7 mRNA was lower than that of HR-HPV DNA (P＜0.01).The positive rate of HPV E6/E7 mRNA for cervical intraepithelial neoplasia Ⅱ+ (CIN Ⅱ+) and CIN Ⅲ + were higher than that of CIN Ⅱ-and CIN Ⅲ-.The sensitivity,specificity,positive predictive value,negative predictive value and accuracy of HPV E6/E7 mRNA in diagnosis of high grades cervical intraepithelial neoplasia (CIN Ⅱ+ and CIN Ⅲ+) were 0.80,0.90,0.79,0.91 and 86.9％,respectively;while those indicators of HR-HPV DNA were 0.82,0.49,0.43,0.85 and 59.4％,The specificity,positive predictive value and accuracy of HPV E6/E7 mRNA weve higher than those of HR-HPV DNA (aⅡ P＜0.05).The results also demonstrated that the HPV E6/E7 mRNA copies were positively correlated with histological severity (r=0.658,P＜0.01).Conclusion The detection of HPV E6/E7 mRNA is of value in predicting the residual/recurrence of ASC-US after cervix conization,indicating that it might be a promising technique in triage of patients with ASC-US after cervical conization.关键词
人乳头状瘤病毒/HPV E6/E7 mRNA/不能明确意义的非典型鳞状上皮细胞/宫颈锥切术Key words
Human papilloma virus/HPV E6/E7 mRNA/Atypical squamous cell of undetermined significance/Cervical conization
