浙江医学2018，Vol.40Issue(7)：693-695,700,4.DOI:10.12056/j.issn.1006-2785.2018.40.7.2017-848
Th17细胞在过敏性哮喘发病中的作用及其与Notch信号通路的关系
Role of Th17 in pathogenesis of allergic asthma and its relation to Notch signaling pathway
摘要
Abstract
Objective To investigate the role of T helper cell 17 (Th17) in the pathogenesis of allergic asthma and its relation to Notch signaling pathway.Methods Forty children with allergic asthma and 40 healthy children (controls) were enrolled in the study.Flow cytometry was used to detect the percentage of Th17cell.Quantity real-time polymerase chain reaction (qRT-PCR)was used to detect the mRNA expression of Th17 related transcription factor retinoid-related orphan nuclear receptor γ t (ROR γt),the mRNA expression of Notch1 and Notch4 in peripheral blood mononuclear cells (PBMC).The concentrations of IL-17 in plasma were tested by enzyme linked immunosorbent assay (ELISA).The correlations of Notch1 mRNA expression with the percentage of Th17 cell,RORγt expression in PBMC and plasma IL-17 concentration were analyzed.Results There was a significantly increased percentage of Th17 cells in allergic asthmatic children compared to healthy controls(P＜0.01).The mRNA expression of ROR γ t was significantly higher in allergic asthmatic children compared to healthy controls(P＜0.01).The plasma level of IL-17 was significantly higher in asthmatic children (P＜0.05).There was a significant increase of the mRNA expression of Notch1,but no increase in the mRNA expression of Notch4 (P ＞0.05).The mRNA expression of Notch1 was positively correlated with the percentage of Th17 cells,ROR γ t expression and plasma IL-17 levels(r=0.780,0.555 and 0.636,all P＜0.01).Conclusion Th17 may play an important role in the pathogenesis of allergic asthma in children,which may be associated with the Notch1 signaling pathway.关键词
儿童/哮喘/辅助性T细胞17/Notch信号通路Key words
Children/Asthma/Th17/Notch signaling pathway引用本文复制引用
盛安群,钱燕,李昌崇,张雪雅,翁翠叶,张维溪..Th17细胞在过敏性哮喘发病中的作用及其与Notch信号通路的关系[J].浙江医学,2018,40(7):693-695,700,4.基金项目
国家自然科学基金资助项目(81100015、81770030) （81100015、81770030）
浙江省医药卫生科技计划骨干人才项目(2014RCA020) （2014RCA020）
浙江省卫生高层次创新人才资助项目 （）
温州市公益性社会发展(医疗卫生)科技项目(Y20170631) （医疗卫生）
