浙江医学2018，Vol.40Issue(8)：786-791,6.DOI:10.12056/j.issn.1006-2785.2017.40.8.2017-2908
乳腺癌干细胞的树突状细胞疫苗对细胞毒性T淋巴细胞增殖的影响
Dendritic cell vaccine prepared with breast cancer stem-like cells activates cytotoxic T lymphocytes
摘要
Abstract
Objective To investigate the effect of dendritic cell (DC) vaccine prepared with human breast cancer stem cells (BSCs) on the activation of cytotoxic T lymphocytes (CTLs).Methods The BSCs were isolated from breast cancer patients,and the human DCs were stimulated with BSC lysates to prepare the DC vaccine against human BSC.The activity of cytotoxic T cells against BSC in vitro was tested.Results The BSCs expressed high levels of stem cell-associated molecules CD44+/CD24-and CD133+,while adherent cells expressed CD44+/CD24+ and low CD133+.The enriched breast cancer cell spheres exhibited stronger tumorigenicity than adherent cells.DC vaccine prepared with BSC lysates elicited specific T-cell responses against BSC.DC vaccine stimulated Th1 response and induced significant IFN-γ production,which was correlated with the number of activated CTLs.Conclusion The breast cancer cell spheres have stem cell characteristics and strong tumorigenicity.DC inoculation with BSC antigen can elicit effective antigen-specific Th1 response,further activating antigen-specific CTLs and inducing IFN-γ production.关键词
乳腺癌干细胞/细胞毒性T淋巴细胞/树突状细胞Key words
Breast cancer stem cell/Cytotoxic T lymphocytes/Dendritic cells引用本文复制引用
彭莹莹,叶小磊..乳腺癌干细胞的树突状细胞疫苗对细胞毒性T淋巴细胞增殖的影响[J].浙江医学,2018,40(8):786-791,6.基金项目
国家自然科学基金项目(2014A610231) （2014A610231）
