浙江医学2018，Vol.40Issue(8)：796-802,812,后插3,9.DOI:10.12056/j.issn.1006-2785.2017.40.8.2018-339
miR-455-5p在结直肠癌中的表达及生物学特性
Expression of miR-455-5p in colorectal carcinoma and its relation to biological features of cancer
王金秋 1鲁杨 1柯孔亮 2张乐鸣2
作者信息
- 1. 315211 宁波大学医学院
- 2. 宁波市第一医院肛肠科
- 折叠
摘要
Abstract
Objective To investigate the expression of miR-455-5p in colorectal carcinoma and its relation to biological features of cancer.Methods The surgical specimens of cancer tissue and pericancerous mucosa tissue were collected from 40 cases of colorectal carcinoma.The expression of miR-455-5p was determined by qRT-PCR in the cancer tissue and pericancerous tissue.The colorectal cancer SW-480,HCT-116,HT-29 cells were transfected with miR-455-5p-mimic or inhibitor,respectively.The cell growth and proliferation capacity were assayed by Brdu method;the cell apoptosis was tested by flow cytometry;the target genes of miR-455-5p were predicted with bioinformatic analysis.Results The expression of miR-455-5p was down-regulation in colorectal cancer tissue compared with pericancerous tissue.In the colorectal cancer cells transfected with miR-455-5p-mimic,the expression of miR-455-5p was up-regulated,and the cell proliferation and migration were inhibited,and apoptosis was promoted.Bioinformatic analysis showed that PIK3R1 might be the target gene of miR-455-5p.The up-regulation of miR-455-5p inhibited the expression of PIK3R1 mRNA and protein.Conclusion The expression of miR-455-5p is down-regulated in colorectal cancer,indicating that miR-455-5p may inhibit the growth of CRC cells,which may be related to the regulation of PIK3R1 expression.关键词
结直肠癌/逆转录作用/靶基因/生物学特性Key words
Ccolorectal cancer/Reverse transcription/Target gene/Biological characteristics引用本文复制引用
王金秋,鲁杨,柯孔亮,张乐鸣..miR-455-5p在结直肠癌中的表达及生物学特性[J].浙江医学,2018,40(8):796-802,812,后插3,9.
