作物学报2018，Vol.44Issue(5)：642-649,8.DOI:10.3724/SP.J.1006.2018.00642
普通菜豆中烟草水杨酸结合蛋白2同源基因的鉴定及表达特征分析
Identification and Expression Analysis of Likely Orthologs of Tobacco Salicylic Acid Binding Protein 2 in Common Beans
摘要
Abstract
Salicylic acid can induce the systematic resistant response in plants, and salicylic acid-binding protein 2 is an important esterase in the regulation of salicylic acid content in plant cells. In this study, we used the bioinformatics method to search the orthologous genes of tobacco salicylic acid-binding protein 2 in common bean,which were designated as PvMES1-PvMES7,then analyzed the changes of MES activity and free SA accumulation in plant roots.The expression level of 7 PvMES genes in suscep-tible varieties BRB130 and Baidaodou,resistant varieties Heiyundou and 260205 infected by Fusarium oxysporum f.sp.phaseoli isolate FOP-DM01 was detected by Real time PCR,showing that the expression of PvMES1,PvMES3,PvMES4,PvMES5,and PvMES6 was significantly increased at three days after FOP-DM01 infection,with most significant increase of 7.6 folds and 5.6 folds for PvMES5 gene in Heiyundou and PvMES1 gene in 260205 at 0 day,respectively.The MES activity and free SA content in roots of Heiyundou and 260205 were also improved, which activated the relevant defense response reaction mediated by SA in the host. The results of this study provide a theoretical basis for the resistance molecular breeding of Fusarium wilt in common beans.关键词
普通菜豆/镰孢菌枯萎病/水杨酸结合蛋白2/直系同源基因/系统获得性抗性Key words
common bean/Fusarium wilt/salicylic acid binding protein 2/orthologous genes/system acquired resistance引用本文复制引用
薛仁风,王利,丰明,葛维德..普通菜豆中烟草水杨酸结合蛋白2同源基因的鉴定及表达特征分析[J].作物学报,2018,44(5):642-649,8.基金项目
本研究由国家自然科学基金项目(31401447),辽宁省博士科研启动基金(201501113),特色粮油新品种选育及优质高效生产技术集成与示范(2018416023)和国家现代农业产业技术体系建设专项(CARS-09-08Z)资助.This work was supported by the National Natural Science Foundation of China(31401447),the Liaoning Doctor Startup Foundation(201501113),the New Varieties Breeding,High Quality and Effective Technology Integration and Demonstration of Special Grain and Oil Crop(2018416023),and the China Agriculture Research System(CARS-08-Z8). （31401447）
