作物学报2018，Vol.44Issue(5)：672-685,14.DOI:10.3724/SP.J.1006.2018.00672
玉米种子萌发相关性状的全基因组关联分析
Genome-wide Association Studies of Seed Germination Related Traits in Maize
摘要
Abstract
Germination is important for seed emergence, which has significant impact on maize yield. To reveal the genetic mechanism of maize seed germination, we investigated six traits related to seed germination using 467 diverse inbred lines. The genome-wide association studies (GWAS) between the six traits and 1.25M SNPs were implemented in three different models (Q model, K model, and Q+K model). The K model was much better than the other two models for weight before imbibition, volume before imbibition, weight after imbibition, volume after imbibition and volume of imbibition. While weight of imbibition trait could be well evaluated by Q+K model. In total, 15 SNPs were significantly associated with the six traits by the optimal model. These SNPs correspond to six QTLs, including five QTLs co-located in different biological replications. The six QTLs were lo-cated on chromosomes 3, 6, 7, and 10. The single SNP could explain 5.09%-7.85% variation of phenotype. Genes within or nearby most significant SNP were selected as candidates, and six candidate genes were identified for seed germination related traits in the six loci. Among these genes, GRMZM2G148411 encoding a TLD-domain calcium ion binding protein according to the annotations associated with weight after imbibition, volume after imbibition and volume of imbibition might be a signal molecule regulating seed germination and dormancy. The QTLs identified in this study are useful for developing functional mark-ers and elucidating the genetic basis of seed germination.关键词
玉米/种子萌发/全基因组关联分析/候选基因Key words
maize/seed germination/genome-wide association study/candidate gene引用本文复制引用
田润苗,张雪海,汤继华,白光红,付志远..玉米种子萌发相关性状的全基因组关联分析[J].作物学报,2018,44(5):672-685,14.基金项目
本研究由国家自然科学基金地区科学基金项目(31760389)资助.This study was supported by the Regional Science Foundation of National Natural Science Foundation of China(31760389). （31760389）
