水稻咪草烟抗性的遗传分析及其紧密连锁分子标记的筛选与应用
Genetic Analysis of Imazethapyr Resistance in Rice and the Closely Linked Marker Selection and Application
摘要
Abstract
Cultivation and extension of herbicide-resistant rice can increase the efficiency of weed control and obtain great eco-nomic benefits. Here, Jinjing 818 was identified as an imazethapyr-resistant line, F2population from the cross between Jinjing 818 and the conventional japonica rice Suken 118 was used for genetic analysis and gene mapping of the imazethapyr resistance in rice. The imazethapyr resistance in Jinjing 818 was controlled by a single dominant nuclear gene located between SSR markers RM7413 and RM7426 on the chromosome 2. Through gene-predicting and sequencing in this region, we found that the target gene of imazethapyr,acetolactate synthase gene(ALS),had a nucleotide mutation(G mutated into A)in its coding region,leading to the mutation of serine into asparagine.It could be preliminarily deduced that ALS is the candidate gene for imazethapyr resis-tance.The physical distances between RM7413,RM7426 and ALS were 165 kb,1612 kb,respectively.Jinjing 818 and Nanjing 9108 were used as parents to detect the application potential of RM7413 in molecular marker assisted selection breeding (MAS). On the basis of selecting phenotype and RM7413 in the hybrid and its selfing generation, F7population showed stable imazethapyr resistance, indicating that the great application potential of RM7413 in MAS and breeding for imazethapyr resistant rice.Our results will lay a foundation for breeding herbicide-resistant japonica rice by MAS.关键词
粳稻/分子标记辅助育种/RM7413/金粳818/咪草烟Key words
Oryza sativa subsp.Keng/Molecular marker assisted selection/RM7413/Jinjing 818/Imazethapyr引用本文复制引用
费云燕,杨杰,范方军,王芳权,李文奇,王军,朱金燕,仲维功..水稻咪草烟抗性的遗传分析及其紧密连锁分子标记的筛选与应用[J].作物学报,2018,44(5):716-722,7.基金项目
本项目由国家重点研发计划项目(2017YFD0100403),江苏省现代农业重点研发项目(BE2015355),江苏省农业科学院探索性项目(ZX(17)2014)和江苏省自然科学基金项目(BK20171326)资助?This study was supported by the National Key Research and Development Program(2017YFD0100403),the Jiangsu Province Key Research and Development Program(Modern Agriculture)(BE2015355),the Exploratory Project of the Jiangsu Academy of Agricultural Sciences(ZX(17)2014),and the Project of Jiangsu Province Natural Science Foundation(BK20171326).This study was supported by the National Key Research and Development Program(2017YFD0100403),the Jiangsu Province Key Research and Development Program(Modern Agriculture)(BE2015355),the Exploratory Project of the Jiangsu Academy of Agricultural Sciences(ZX(17)2014),and the Project of Jiangsu Province Natural Science Foundation(BK20171326). （2017YFD0100403）
