作物学报2018，Vol.44Issue(5)：723-736,14.DOI:10.3724/SP.J.1006.2018.00723
甘蔗品种的AFLP和SSR标记鉴定及其应用
Identification of Sugarcane Varieties by AFLP and SSR Markers and Its Application
摘要
Abstract
Genetic diversity and fingerprint of varieties are crucial reference for breeding, cultivar right protection and new cultivar extension. We used nine pairs of AFLP markers and 15 pairs of SSR markers to scan 38 new sugarcane varieties (lines) from the national variety regional testings, obtaining abundant fingerprint data. Among the total 348 AFLP bands, 248 were polymorphic, with a polymorphism rate of 72.26 %. In addition, among the total 180 SSR bands, 176 were polymorphic, with a polymorphism rate of 97.78 %. The genetic similarity coefficients of these 38 new varieties (lines) were distributed from 0.668 to 0.847. We explored the distribution characteristics of genetic similarity coefficients using the boxplot, observing the genetic basis of five series (FN, MT, YZ, YG, and GT) of the 38 new sugarcane varieties (lines) was approximately similar. The clustering analysis manifested that these 38 new sugarcane varieties (lines) were divided into two groups at the genetic similarity coefficient of 0.732, with a subgroup including FN09-2201 and GT06-1492 that had high heterogeneity. Moreover, there was a small subgroup containing ROC22 at the genetic similarity coefficient of 0.770. Except for ROC22, the subgroup also contained FN 07-3206, FN 40, HZ 22, GT 09-12, and LC 07-150. ROC22 has wide adaptability, high yield, high sugar content and other excellent characteristics, other varieties (lines) in the same subgroup should be more likely to have these excellent characteristics and higher extention potential. Finally, through selection of 60 efficient amplification sites in the identification of SSR markers,we constructed the fingerprints of these 38 new sugarcane varieties (lines), which should play an important role in variety identification and variety protection. This study is expected to be directly applied to guide the genetic diversity assessment and molecular fingerprinting identification of sugarcane germplasm, and also to provide references for the extention and layout of these varieties or their utilization as hybrid parents.关键词
甘蔗/分子标记/种质鉴定/群体划分/推广布局Key words
sugarcane/molecular marker/germplasm identification/group division/variety extension引用本文复制引用
汪洲涛,游倩,高世武,王春风,李竹,马晶晶,阙友雄,许莉萍,罗俊..甘蔗品种的AFLP和SSR标记鉴定及其应用[J].作物学报,2018,44(5):723-736,14.基金项目
本研究由国家现代农业产业技术体系建设专项(CARS-17)和引进国际先进农业科学技术计划(948计划)项目(2014-S18)资助.This study was supported by China Agriculture Research System(CARS-17)and the Program of Introducing International Super Agricultural Science and Technology(948 Program)(2014-S18). （CARS-17）
