作物学报2018，Vol.44Issue(5)：783-789,7.DOI:10.3724/SP.J.1006.2018.00783
高粱种子萌发过程中超微弱光子辐射的特征
Characteristics of Ultra-weak Photon Emission from Sorghum Seeds during Germination
摘要
Abstract
Ultra-weak photon radiation from germinated seed includes spontaneous photon emission of germinated seeds in the dark and delayed photon radiation induced by external light. In order to study dynamical feature and biological significance of ultra-weak photon radiation from sorghum during germination, 50 μg mL-1protein synthesis transcription inhibitor actinomycin D (AMD), 100 μg mL-1protein synthesis translation inhibitor cycloheximide (CHM) and 650 μg mL-1respiration inhibitor were adopted to treat germinated sorghum seeds separately. There was a positive correlation (correlation coefficient was 0.93121) be-tween the spontaneous photon emission intensity of germinated sorghum and seed fresh mass. AMD and CHM partially inhibited while NaN3almost completely inhibited the increase of fresh mass of sorghum seeds in germination process. It indicated that the spontaneous photon emission intensity from germinated sorghum seed can be taken as a signal of germination status from which RNA/DNA synthesis reaction is one of the sources and respiratory metabolism is the main source. The characteristic of delayed photon emission from germinated sorghum seeds can be expressed quantitatively by dynamical parameters such as initial photon number, coherence time and the integrated intensity of delayed photon emission. The cell metabolism and its changes in germina-tion process of sorghum seeds can be understand and evaluated through a real-time, sensitive and non-destructive analysis of the integrated intensity of delayed photon emission from germinated sorghum seeds.关键词
高粱/萌发/超微弱光子辐射/蛋白质合成抑制剂/呼吸代谢抑制剂/无损检测Key words
sorghum/germination/ultra-weak photon radiation/protein synthesis inhibitor/respiratory metabolic inhibitor/non-destructive test引用本文复制引用
赵燕燕,习岗..高粱种子萌发过程中超微弱光子辐射的特征[J].作物学报,2018,44(5):783-789,7.基金项目
本研究由国家自然科学基金项目(31471412,51277151)资助.This study was supported by the National Natural Science Foundation of China(31471412,51277151). （31471412,51277151）
