植物研究2018，Vol.38Issue(3)：367-376,10.DOI:10.7525/j.issn.1673-5102.2018.03.008
两种氮素形态对药用植物三叶青中黄酮类活性成分积累的影响及相关分子机制的初步探讨
Preliminary Study on the Effects of Two Different Nitrogen Forms on Flavonoids Accumulation and Related Molecular Mechanism in Medicinal Plant Tetrastigma hemsleyanum
摘要
Abstract
The effects of two different nitrogen(N) forms(nitrate-N and ammonium-N) on the accumulation of flavonoids(procyanidin B1,rutin,kaempferol-3-O-rutinoside,quercetin,isoquercitrin and kaempferol) in the endangered medicinal plant Tetrastigma hemsleyanum (from Zhejiang Province) were examined through potted experiment under shady condition (30％ natural light),and its influence mechanism was also discussed.The flavonoids content was relatively higher in the root and leaf of T.hemsleyanum under nitrate-N supply than ammonium-N supply.The levels of transcription and expression of phenylalanine ammonia-lyase (PAL) and chalcone synthase(CHS) which are related to flavonoids synthesis were relatively higher in the root and leaf of T.hemsleyanum under nitrate-N supply than ammonium-N supply.Besides,the enzyme activities of PAL and CHS were also higher in the root and leaf of T.hemsleyanum under nitrate-N supply than ammonium-N supply.The PAL-specific inhibitor(AOA) could significantly inhibit the enzyme activity of PAL(P ＜0.05) and lead to a very significant decrease in flavonoids content(P ＜0.01).In conclusion,nitrate-N supply is more beneficial to flavonoids synthesis in T.hemsleyanum,which mainly enhances the flavonoids synthesis by increasing the gene expression and activity of key enzymes in PAL metabolic pathway.关键词
氮素形态/氮同化/黄酮类/三叶青Key words
nitrogen forms/nitrogen assimilation/flavonoids/Tetrastigma hemsleyanum分类
农业科技引用本文复制引用
张强,刘一,吴晓敏,束良佐,宋运贤,陈楚,龙民慧..两种氮素形态对药用植物三叶青中黄酮类活性成分积累的影响及相关分子机制的初步探讨[J].植物研究,2018,38(3):367-376,10.基金项目
国家自然科学基金(31500594,31572202) （31500594,31572202）
安徽省教育厅重点项目(KJ2014A227,KJ2015A302) （KJ2014A227,KJ2015A302）
安徽高校优秀青年人才支持计划重点项目(gxyqZD2016415)National Natural Science Foundation of China(31500594,31572202) （gxyqZD2016415）
Natural Science Foundation from Educational Commission of Anhui Province(KJ2014A227,KJ2015A302) （KJ2014A227,KJ2015A302）
Key project of supporting program for outstanding youth talents of Anhui provincial universities (gxyqZD2016415) （gxyqZD2016415）
