植物研究2018，Vol.38Issue(3)：391-398,8.DOI:10.7525/j.issn.1673-5102.2018.03.011
碳源、生长素及诱导子对木豆不定根生长和次生代谢产物合成的影响
Effect of Carbon Source, Auxin and Elicitor on the Growth and Synthesis of Secondary Metabolites of Adventitious Roots of Cajanus cajan (Linn.) Millsp.
摘要
Abstract
Using 1/2MS as basic medium,we studied sucrose,IBA,jasmonic acid methylester(MJ) and salicylic acid(SA) on the influence of biomass and synthesis of secondary metabolites of adventitious root of Cajanus cajan(Linn.) Millsp..The content of sucrose had a significant influence on the biomass and synthesis of secondary metabolites.When the content of sucrose was 30 g · L-1,the amount of biomass and the content of secondary metabolites of adventitious root of C.cajan (Linn.) Millsp were the maximum value.The low concentration of IBA is beneficial to the growth of adventitious root of C.cajan (Linn.) Millsp.and the accumulation of secondary metabolites.However,the high content of IBA shows inhibition.When the content of IBA was 0.1 mg · L-1,biomass,genistein and apigenin were maximum,1.1,1.1 and 2.8 times as control group respectively.MJ with 0-200 μmol · L-1 almost had no effect on adventitious roots growth (P ＞ 0.05),but it had a significant effect on the synthesis of secondary metabolites.When the content of MJ was 100 μmol · L-1,genistein and apigenin were maximum,respectively,1.9 and 2.1 times as the control group.The growth of adventitious roots and the synthesis of genistein were inhibited by SA.However,SA had a certain effect on the synthesis of apigenin.When the content of SA was 100 μmol · L-1,the content of apigenin was maximum,1.5 times as the control group.Suspension culture of adventitious roots of C.cajan (Linn.) was an effective method to obtain the secondary metabolites,and will provide a good way of thinking for large-scale production of genistein and apigenin.关键词
木豆/不定根/生物量/次生代谢产物Key words
Cajanus cajan (Linn.)/adventitious roots/biomass/secondary metabolites分类
农业科技引用本文复制引用
范艳敏,党士坤,王文杰,王慧梅..碳源、生长素及诱导子对木豆不定根生长和次生代谢产物合成的影响[J].植物研究,2018,38(3):391-398,8.基金项目
黑龙江省自然科学基金(C2015051) （C2015051）
中央高校基本业务基金(2572017EA03)Natural Science Fund of Heilongjiang Province(C2015051) （2572017EA03）
Special funds for basic research of central university(2572017EA03) （2572017EA03）
