植物研究2018，Vol.38Issue(3)：415-421,7.DOI:10.7525/j.issn.1673-5102.2018.03.014
基于转录组测序的慈竹笋木质素基因筛选及其表达分析
Transcriptome-wide Identification of Lignin Gene Families of Bambusa emeiensis Shoots and Their Differential Expression
摘要
Abstract
Bambusa emeiensis is a good fiber raw material,but the presence of lignin negatively affects bamboo pulp production.The 351 lignin biosynthesis-related unigenes of B.emeiensis were indentified through BLASTp combined with phylogenetic analysis,based on the transcriptome data of B.emeiensis shoots with 10 cm 150 cm height.Among them,the numbers of transcripts including 51 laccase (LAC),374CL,26PAL and 25CAD were found,and all these transcripts having more abundant in B.emeiensis than other reported bamboo species.RNA-seq and real-time quantitative PCR(RT-qPCR) analysis were used to investigate the differential expression of these lignin genes in bamboo shoots at the different elongation stages,and the relationship between lignin gene expression and lignin amount was further determined.The results revealed that with the elongation of shoots,the expression levels of 16 lignin biosynthesis genes,including 2 PAL,5 CCR,34CL,2CAD and 4LAC,were up-regulated,indicating their roles in developmental lignin accumulation of B.emeiensis.关键词
慈竹/笋/木质素生物合成/差异表达Key words
Bambusa emeiensis/bamboo shoots/lignin biosynthesis/differential expression分类
农业科技引用本文复制引用
蒋海港,王树刚,刘阳,沈彦合,李祥宁,曹颖..基于转录组测序的慈竹笋木质素基因筛选及其表达分析[J].植物研究,2018,38(3):415-421,7.基金项目
四川省大学生创新创业训练计划资助项目(16cxcy13) （16cxcy13）
西南科技大学实验技术研究项目(16syjs19) （16syjs19）
西南科技大学生命科学基地班创新实践项目(SMJDB009)Project(16cxcy13) supported by Sichuan's Training Program of Innovation and Enterpreneurship for Undergraduate （SMJDB009）
Project (16syjs19) supported by Experimental Technique Exploiting Program of Southwest University of Science and Technology （16syjs19）
Project (SMJDB009) supported by Innovative Practice Program of Southwest University of Science and Technology （SMJDB009）
