安徽农业科学2018,Vol.46Issue(14):47-50,85,5.
2个番木瓜品种组培苗成苗率研究
Study on the Survival Rate of Two Varieties of Papaya Plantlets for Tissue Culture Seedling
摘要
Abstract
[Objective]To find a breeding technique for papaya with high seedling rate and good quality commercial seedling. [Method]'suoluo' variety was selected as the experimental material,and MS was used as the basic culture medium.Growth regulator combinations that contained IBA,NAA,KT was designed,and the orthogonal experiment was used with the different ratio of growth regulator for rooting medium screening. [Result]The optimum combining ratio of root stimulating hormone was IBA 0.75 mg/L,NAA 0.05 mg/L and KT 0.01 mg/L,and the rooting percentage was 66.7%,but'mihong' varietie did not achieve the same effect. The papaya seedlings were respectively soaked in four different nutrient root water for 6 h by contrast experiment. It was determined the rooting water concentration was 50 mg/L to get the high-est rate of seedling formation,and reaching 86.1%. Fixed the best rooting water concentration,and the soaking time of 5 different gradients was tested. Without regard for time cost,finally,the highest percentage of seedlings was 8 hours for soaking,reaching 87.5%. [Conclusion] The survival rates of two varieties of papaya were both almost the same.关键词
番木瓜/组培苗/正交试验/生根率/成苗率Key words
Carica papaya L./Tissue culture seedling/Orthogonal experiment/Rooting rate/Seedling rate分类
农业科技引用本文复制引用
谢智旭,沈文涛,言普,庹德财,黎小瑛,周鹏..2个番木瓜品种组培苗成苗率研究[J].安徽农业科学,2018,46(14):47-50,85,5.基金项目
中国热带农业科学院热带生物技术研究所基本科研业务费专项(1630052016005). (1630052016005)