安徽农业科学2018，Vol.46Issue(14)：109-112,4.
山葡萄北冰红VvCOR413-I1基因克隆及生物信息学分析
Cloning and Bioinformatic Analysis of VvCOR413-I1 Gene from Beibinghong Amur Grape
摘要
Abstract
[Objective]The cold-tolerant gene COR413 was cloned from the Beibinghong Amur grape,and its function was inferred through bioin-formatics analysis.[Method]The RT-PCR method was used to clone COR413,a cold-tolerant gene from the Beibinghong Amur grape,and its mo-lecular characteristics were analyzed by bioinformatics to infer the function of COR413 gene.[Result]The ORF of COR413 was 630 bp and the deduced amino acid sequence was 230 amino acids.Furthermore,its protein had a MW of 59.18 kD and an isoelectric point(pI) of 10.74.The structure of the protein was dominated by α-helix and elongation chain,followed by random coil,the proportion of β-turn was the smallest,con-taining 4 transmembrane domains.Multiple sequence alignment showed that the amino acid sequence similarity of COR413 with other plants was 63.00% to 68.33%.[Conclusion]The study laid the foundation for an in-depth understanding of VvCOR413-I1 function.关键词
冷适应蛋白基因(COR413)/山葡萄北冰红/克隆/生物信息学分析Key words
Cold-regulated gene (COR413)/Beibinghong Amur grape/Clone/Bioinformatic analysis分类
农业科技引用本文复制引用
王学娟..山葡萄北冰红VvCOR413-I1基因克隆及生物信息学分析[J].安徽农业科学,2018,46(14):109-112,4.基金项目
内蒙古民族大学科学研究项目(NMDYB17152). （NMDYB17152）
