安徽农业科学2018，Vol.46Issue(14)：113-115,3.
一株野生大型真菌的菌种分离及ITS序列鉴定
Isolation and Identification of ITS Sequences of a Wild Macrofungi
摘要
Abstract
[Objective]To find suitable strains of wild edible fungi cultivated under poplar forests. [Method]Wild macrofungus under Populus alba forest collected from Mongolia Autonomous County,Fuxin City,Liaoning Province was isolated and the effects of different separation sites on mycelium growth were studied. The rDNA ITS sequences of mycelium were studied.[Result]The junction between the cap and stipe was the best separation site for the fungus. The rDNA ITS fragment was amplified by PCR, the length of the amplified product was 692 bp. The Blast comparison was performed between NCBI and a known strain in GenBank. The Query cover between sequence and Agaricus bitorquis (Quél.) Sacc was 97% to 100%,the idents were all 99%.[Conclusion]The identified strains belong to mushroom family,mushroom genus and Agaricus bitorquis.The study can provide the preparation of strains for the nest stage of poplar-edible fungus compound management.关键词
杨树/野生大型真菌/菌种分离/ITS序列鉴定/大肥菇Key words
Poplar/Wild macrofungi/Isolation of strains/ITS sequence identification/Agaricus bitorquis分类
农业科技引用本文复制引用
宋立志,冯连荣,梁德军,矫丽曼,张妍..一株野生大型真菌的菌种分离及ITS序列鉴定[J].安徽农业科学,2018,46(14):113-115,3.基金项目
国家重点研发计划课题(2016YFD0600401) （2016YFD0600401）
辽宁省农业攻关及成果产业化项目(2014207011). （2014207011）
