波谱学杂志2018，Vol.35Issue(2)：198-203,268,7.DOI:10.11938/cjmr20182630
JAK/STAT通路抑制剂对C57BL/6小鼠血清代谢组影响的1H NMR研究
1H NMR Study on Serum Metabonomic Alterations Induced by JAK/STAT Pathway Inhibitor in C57BL/6 Mice
摘要
Abstract
JAK/STAT pathway inhibitor has been used in clinic trial for several diseases. However, the adverse effect of systematic administration of JAK/STAT pathway inhibitors on cellular function is largely unknown. In the present study, alteration of metabolites in C57BL/6 mice serum were characterized using metabonomic analysis after WP1066 (a JAK/STAT pathway inhibitor) treatment (20 mg/kg.bw, once every 2 days for 2 weeks). 1H NMR spectroscopy was used to detect the effect of WP1066 on metabolites component, and the metabolite profile were analyzed by principle component analysis (PCA) and orthogonal partial least squares (OPLS) analysis. Results showed that the level of N-methylnicotinamide was significantly downregulated, while cis-aconitate, oxaloacetate and acetamide were significantly upregulated in mice serum in response to JAK/STAT pathway inhibition. The above metabolic changes are associated with alterations in energy metabolism, indicating that parameters associated with metabolism unbalance may be used for monitoring the efficiency and adverse effect of JAK/STAT pathway inhibitor.关键词
核磁共振(NMR)/JAK/STAT通路抑制剂/代谢组学/能量代谢Key words
nuclear magnetic resonance (NMR)/JAK/STAT pathway inhibitor/metabonomics/energy metabolism分类
数理科学引用本文复制引用
赵欣,肖雄杰,高东莉,张先荣..JAK/STAT通路抑制剂对C57BL/6小鼠血清代谢组影响的1H NMR研究[J].波谱学杂志,2018,35(2):198-203,268,7.基金项目
国家自然科学基金资助项目(81573515). （81573515）
