草业科学2018，Vol.35Issue(5)：1138-1144,7.DOI:10.11829/j.issn.1001-0629.2017-0494
不同紫花苜蓿杂交组合对冻害胁迫的生理响应
Physiological responses of different alfalfa hybrid combinations to freezing stress
摘要
Abstract
Freezing inj ury is the main limiting factor for alfalfa in northeast China.The screening of breeding materials that can resist freeze—thaw stress is of great significance for improving the yield and quality of alfal-fa.In the present study,five different combinations of alfalfa(Medicago sativa)were selected as test materi-als,which were subj ected to three different treatment stages:cold resistance training stage,freezing stage,and turning green stage.soluble protein(SP),soluble sugar(SS),proline(Pro),and malondialdehyde(MDA)con-tents,and superoxide dismutase(SOD)activity were measured.The results showed that the SP content had an overall increasing trend and was significantly higher in each test material than in the control group.The SS con-tent fluctuated and was significantly lower in all test materials than in the control group during the cold resist-ant stage.The SS content was significantly higher in all test materials than the control group during the frozen stage,and the Pro content generally increased.MDA contents in the Ⅱ,Ⅲ,and Ⅴ hybrid combinations were lower than that in the control group,although this difference was not significant.SOD activities first increased and then decreased,with the contents during the other phases significantly higher than that in the control group.Membership function analysis showed that the order of cold resistance in the hybrid combinations wasⅤ>Ⅲ>Ⅰ>Ⅱ>Ⅳ.关键词
紫花苜蓿/杂交组合/抗寒锻炼/冻害胁迫/生理响应/隶属函数法/抗寒性评价Key words
alfalfa/hybrid combination/freezing stress/assessment of cold-tolerance分类
农业科技引用本文复制引用
陈晶晶,王英哲,金艳,郭强,徐博..不同紫花苜蓿杂交组合对冻害胁迫的生理响应[J].草业科学,2018,35(5):1138-1144,7.基金项目
国家自然科学基金"基于简单重复序列的紫花苜蓿细胞质雄性不育恢复基因的定位研究"(31402125) （31402125）
国际科技合作专项项目课题"重要牧草资源抗寒性评价鉴定及创新利用"(2015DFR30570-2) （2015DFR30570-2）
公益性行业(农业)科研专项项目课题"东北寒冷半湿润区苜蓿高效种植技术研究与示范"(201403048-0400) （农业）
