草业科学2018，Vol.35Issue(5)：1315-1322,8.DOI:10.11829/j.issn.1001-0629.2017-0580
草类植物微生物实验室的杂菌种类及其来源
The contaminant microorganism species of a grass plant microbiology laboratory and their source
摘要
Abstract
In this research proj ect,the potato dextrose agar(PDA)plate exposure method was used to collect microorganisms from a sterile room and from a conventional operation room in a microbiology laboratory.The main strains were identified by morphological and molecular morphology.Changes in the bacterial colony after sterile operations,such as spraying with alcohol or wiping the table,were observed.Research showed that the laboratory had a total of 1 1 kinds of contaminating microorganism,including Bacillus amyloliquefaciens,Di-etzia maris,and Arthrobacter globiformis.Altogether,there were eight kinds of bacteria and three kinds of fungi,namely Penicillium sp.,Peziza ostracoderma,and Cladosporium cladosporioides.Among them,the dominant strains were Kocuria rosea,A.globiformis,and D.maris,with the colonies numbering 28,28,and 23,respectively.The study found that the kinds of microorganism of sterile room where placed three ultra clean bench more than conventional operation room,and contains all kinds of conventional operation room.The study indicated that the microbes in the conventional operation room came from the sterile room.There was a large difference between the microorganism species of the laboratory and the common microbial species in the air,indicating that the microbes of the sterile room may have been introduced by improper operations during microbial research.After carrying out sterile operations,the contaminant microorganisms of the inside and the outside of the ultra—clean benches were reduced by 64% and 9%,respectively.The experiments show that wi-ping out the ultra-clean bench and spraying alcohol can effectively reduce the pollution of microbial cultures. Based on the microbial source,it is necessary to standardize the operation and reduce the air flow between the laboratory and outside.This study provides a scientific basis for the detection,prevention,and control of mi-crobial contamination in a microbiology laboratory.关键词
微生物污染/检测/鉴定/来源Key words
microbial contamination/detection/identification/resource分类
农业科技引用本文复制引用
胡进玲,古丽君,曹师,成凤花,李彦忠..草类植物微生物实验室的杂菌种类及其来源[J].草业科学,2018,35(5):1315-1322,8.基金项目
公益性行业(农业)科研专项经费项目(201303057) （农业）
国家现代农业产业技术体系(CARS-34) （CARS-34）
云南省高端科技人才引进项目(2012HA012) （2012HA012）
中央高校基本科研业务费(lzujbky-2017-k02) （lzujbky-2017-k02）
高等学校学科创新引智计划(B12002) （B12002）
