重庆医学2018，Vol.47Issue(14)：1841-1844,1849,5.DOI:10.3969/j.issn.1671-8348.2018.14.001
高压氧治疗对高原脱习服症大鼠主要脏器超微结构的影响
Effects of hyperbaric oxygen treatment on ultrastructure of rats with plateau deacclimatization
摘要
Abstract
Objective To research the effects of hyperbaric oxygen (HBO) on the ultrastructure of heart,brain and lung in rats with plateau deacclimatization.Methods Forty-mine SD rats were selected.Fortyfour SD rats were routinely fed in a hypobaric hypoxia chamber at the simulated altitude of 5 000 m for 3 months.Nine rats died in the hypobaric hypoxia chamber.After coming out from the chamber,35 survival rats were randomly divided into the high altitude control group (instantly after out of the hypobaric hypoxia chamber),HBO group Ⅰ,Ⅱ and Ⅲ (7 days,1 months and 2 months after HBO treatment) and control groups Ⅰ,Ⅱ and Ⅲ according to the body mass,5 cases in each group;the HBO groups were treated by HBO for 7 d.The control group Ⅰ,Ⅱ and Ⅲ were synchronously fed in normal pressure and oxygen environment after HBO treatment.The rats in all groups were synchnonously sacrificed,the other 5 SD rats as the plain control group were fed in hormal pressure oxygen environment.The frontal cortex,heart and lung tissues were taken out for observing the ultrastructure changes by optical microscopy and electron microscopy.Results Hypoxia induced mitochondria swelling and ridge breakage in cranial nerves cells,swelling of mitochondria and peripheral structures,myocardial cells cloudy swelling,vacuolar degeneration,muscle fibers irregular arrangement,inflammatory cell infiltration and vasodilatation.The alveolar septm was broadened,blood capillaries were dilated and congestive,alveolar space was filled with pink transudation,inflammatory cells were infiltrated,and alveolar wall was incrassated.After HBO treatment,the tissue ultrastructure injurys were significantly improved compared with the control group,the recovery in the HBO group Ⅱ was significantly better than that in the HBO group Ⅰ,and the HBO group Ⅲ was significantly better than the HBO group Ⅱ,which recovered to plain level after 2 months.Conclusion HBO treatment has the effects for improving cranial nerves injury in hypoxia rat,promotes cellular function recovery,decreases hypoxia induced pulmonary hypertension,and has the protective effect on ischemic myocardium.关键词
高原脱习服症/高压氧/组织超微结构Key words
plateau deacclimatization/hyperbaric oxygen/tissue ultrastructure分类
医药卫生引用本文复制引用
崔宇,吴佩锋,徐钢,崔建华..高压氧治疗对高原脱习服症大鼠主要脏器超微结构的影响[J].重庆医学,2018,47(14):1841-1844,1849,5.基金项目
国家科技支撑计划项目(2009BAI85B05) （2009BAI85B05）
高原医学教育部重点实验室资助项目(2011JSGY05) （2011JSGY05）
全军“十一五”医药卫生科研基金资助项目(06MA091). （06MA091）
