重庆医学2018，Vol.47Issue(14)：1878-1881,4.DOI:10.3969/j.issn.1671-8348.2018.14.010
FBP1在肾透明细胞癌组织中的表达变化及意义
FBP1 expression change in renal clear cell carcinoma tissue and its significance
摘要
Abstract
Objective To investigate the expression of FBP1 in human renal clear cell carcinoma and paracancerous tissue and its effect and clinical significance in the carcinogenesis,progression and prognosis of renal cancer.Methods The paraffin sections from 118 patients with renal clear cell carcinoma treated by surgical resection and fresh specimens from 40 patients with renal clear cell carcinoma were selected.The expressions of FBP1 protein and mRNA in renal cancer and paracancerous tissues(negative incisal edge) were detected by adopting the immunohistochemistry(IHC),Western blot and RT-PCR.Its correlation with clinicopathologic characteristics and prognosis of the patients was analyzed.Results The IHC result,found that strong positive expression of FBP1 protein could be seen in 78.81％ (93/118) of cancer-adjacent tissues,while only 39.83％ (47/118) of renal cancer tissues had positive expression.Western blot found that the expression positive rate of FBP1 in renal cancer tissue was significantly decreased compared with corresponding cancer-adjacent tissues (P＜0.01).RT-PCR found that the FBP1mRAN expression level in cancer-adjacent tissues was also significantly higher than that in renal cancer tissue(P＜0.05).The FBP1 low expression was significantly correlated with the clinical stage,pathologic grade,UISS risk coefficient and recurrence(P＜0.05),and had no relation with the age,gender,symptoms,tumor size,location,tumor necrosis,vascular invasion and adrenal involvement(P＞0.05).The 5-year survival rate in renal cancer patients with FBP1 positive expression was higher than that in the patients with FBP1 negative expression(P＜0.05).Conclusion FBP1 and protein are lowly expressed in renal cancer tissue,are correlated with occurrence and development of renal cancer,and may become one of candidate markers of renal cancer prognosis.关键词
肾透明细胞癌/果糖1；6二磷酸酶1/基因表达Key words
renal clear cell carcinoma/fructose-1,6-bisphosphatase/gene expression分类
医药卫生引用本文复制引用
洪正东,朱安义,王艳华,史子敏,胡映波..FBP1在肾透明细胞癌组织中的表达变化及意义[J].重庆医学,2018,47(14):1878-1881,4.基金项目
江西省教育厅课题基金资助项目(GJJ11337) （GJJ11337）
江西省卫生厅课题基金资助项目(20141074). （20141074）
