重庆医学2018，Vol.47Issue(15)：1986-1989,4.DOI:10.3969/j.issn.1671-8348.2018.15.003
IL-1β、IL-6及下游IDO激活与大鼠抑郁行为的关系研究
Relationship between activation of IL-1β,IL-6 and downstream IDO activation with rat depression behaviors
摘要
Abstract
Objective To investigate the relationship between the rat depression behaviors induced by chronically unpredicted stress (CUS) with interleukin-1β (IL-1β),interleukin-6 (IL-6) protein expression and indole-2,3-dioxygenase (IDO) mRNA and protein expression.Methods Thirty male Sprague Dawley (SD)rats were divided into the control group (CG) and model group (MG).The solitary raise combined with CUS 28 d continued stimulus was used to establish the rat depression model.The open field test (OFT) and the force swimming test (FST) were used to evaluate the rat depression behaviors;qRT-PCR was used to determine the mRNA expression of IDO in hippocampus and cortex;Western blot was used to determine the protein expressions of IL-1β,IL-6 and IDO in hippocampus and cortex.Results Compared with the CG group,the OFT score in the rats of the MG group was significantly decreased(P＜0.01),the immobility time in FST was significantly extended (P＜0.00),the expression of IDO mRNA in hippocampus and cortex was significantly increased (P＜0.01) and the protein expression of IL-1β,IL-6 and IDO in hippocampus and cortex was significantly increased (P＜0.05).Conclusion The rat depression behaviors induced by CUS may be associated with the increase of intracerebral inflammatory cytokine expression and inducing IDO over-expression.关键词
抑郁症/慢性不可预见刺激/炎症因子/吲哚胺2,3-双加氧酶Key words
depressive disorder/chronically unpredicted stress/cytokines/indoleamin-2,3-dioxygenase分类
医药卫生引用本文复制引用
伏箫燕,李海燕,崔婷,蒋青松,邱红梅..IL-1β、IL-6及下游IDO激活与大鼠抑郁行为的关系研究[J].重庆医学,2018,47(15):1986-1989,4.基金项目
国家自然科学基金资助项目(31400881) （31400881）
重庆市教委科学技术项目(KJ1400208). （KJ1400208）
