重庆医学2018，Vol.47Issue(15)：1993-1996,4.DOI:10.3969/j.issn.1671-8348.2018.15.005
两种方法建立SD大鼠黄褐斑模型的比较实验研究
Comparative experiment study of two kinds of methods for establishing SD rat chloasma model
摘要
Abstract
Objective To comparatively analyze the SD rat chloasma model established by using the two methods of progesterone injection and ultraviolet radiation.Methods The rat chloasma model was established by adopting the high and low doses of progesterone injection and ultraviolet irradiation.The plaque area was observed and the skin section was observed by light microscope.The SOD and MDA levels closely related with chloasma melanin deposition were detected.Results The significant pigment deposition on the back skin in the high dose progesterone group was seen by naked eye and the scattered pigment deposition was seen in the low dose progesterone group;significant pigment deposition could be found in the ultraviolet group.Compared with the control group,the MDA level of various tissues in the high dose progesterone group was increased,while the SOD level was decreased,serum and skin SOD level in the low dose progesterone group was decreased,the difference was statistically significant(P＜0.05).Serum and skin SOD level in the high dose ultraviolet group was decreased,while the MDA level was increased,the skin SOD level in the low dose ultraviolet group was decreased(P＜0.05),but the difference between the two groups was not statistically significant (P＞0.05).The melanin grains were heaped up as the small black body under light microscope,which in the progesterone group showed the clutter distribution,the difference between the high dose group and low dose group was significant;which in the ultraviolet group showed the linear dense arrangement and the intergroup had no significant difference.Conclusion Progesterone injection and ultraviolet irradiation all can successfully establish the SD rat chloasma model,and the constructing model effect and stability of ultraviolet irradiation are better.关键词
黄褐斑/紫外线/黄体酮/动物模型/大鼠,sprague-dawleyKey words
chloasma/ultraviolet ray/progesterone/animal model/rats, sprague-dawley分类
医药卫生引用本文复制引用
邓志博,何施逸,王乾力,李韫韬,胡乔莉,杜永洪..两种方法建立SD大鼠黄褐斑模型的比较实验研究[J].重庆医学,2018,47(15):1993-1996,4.基金项目
重庆市基础科学与前沿技术研究专项(csct2016jcyjA0098). （csct2016jcyjA0098）
