重庆医学2018，Vol.47Issue(15)：2011-2015,5.DOI:10.3969/j.issn.1671-8348.2018.15.010
非阻塞性无精症患者血清miRNA表达谱分析研究
Analysis study of serum miRNA expression profile in patients with non-obstructive azoospermia
摘要
Abstract
Objective To analyze the serum miRNA expression levels in non-obstructive azoospermia (NOA) patients and healthy sperm donors.Methods Serum miRNA levels in NOA patients and healthy sperm donors were analyzed by adopting the miRNA expression profiles chip.The data were processed and an alyzed by using the GenePix proV6.0 software to find out the differentially expressed miRNA,then the difference was verified by RQ-PCR,finally the bioinformatic software was utilized to predict the miRNA target gene.Results Compared to healthy sperm donors,71 cases of NOA had miRNAs expression difference,miRNA expression were increased in 47 cases and miRNAs expression was decreased in 24 cases.Moreover,Realtime PCR analysis verified the chip accuracy.The bioinformatic software target gene prediction showed that the potential target gene of these differential miRNA were involved in spermatogenesis.Conclusion The specific miRNA exists in serum miRNA of NOA,which helps to study the molecular mechanism of spermatogenesis.关键词
非阻塞性无精症/血清/微RNAs/表达谱Key words
non-obstructive azoospermia/serum/microRNAs/expression profile分类
医药卫生引用本文复制引用
赵文忠,罗招凡,傅文婷,吴瑞珊,钟安,周雨..非阻塞性无精症患者血清miRNA表达谱分析研究[J].重庆医学,2018,47(15):2011-2015,5.基金项目
广东省自然科学基金资助项目(2014A030313721) （2014A030313721）
广东省医学科研基金资助项目(A2017612). （A2017612）
