动物医学进展2018，Vol.39Issue(4)：19-23,5.
一株H3N8亚型马流感病毒的鉴定及HA基因序列分析
Identification and HA Gene Sequence Analysis of a H3 N8 Subtype Equine Influenza Virus Isolate
摘要
Abstract
In April 2016,a suspect equine influenza case occurred in a horse ranch in Beijing.To make a defi-nite diagnosis of the etiological agent,virus isolation was taken by incubating the nose swabs collected from suffering horses to embryonated eggs,then hemagglutination test,hemagglutination inhibition test,gene sequencing and BLAST were conducted.A strain of equine influenza virus (EIV)named A/equine/Beijing/2016 was isolated from the nose swabs.The results of hemagglutination test,hemagglutination inhibition test and gene sequencing showed that the isolate belongs to H3N8 subtype.Further phylogenetic analysis of HA gene of the isolate indicated that this virus belongs to American EIV,Floride sublineage (clade 2).HA amino acid squence analysis of the isolate A/equine/Beijing/2016 and other EIV isolates in China for the past few years was conducted to find there was an important antigenic drift between A/equine/Beijing/2016 and the EIV vaiccine candidate strain recommaned by OIE.Frequent variations exist in HA gene of EIVand molecular epidemioloical surveillance of EIV need to be strengthened.关键词
H3N8马流感病毒/分离/HA基因/序列分析Key words
H3N8 subtype equine influenza/isolation/HA gene/sequence analysis分类
农业科技引用本文复制引用
王小蕾,段会娟,杨志远,刘立新,赵际成,潘洁,刘月焕,林健..一株H3N8亚型马流感病毒的鉴定及HA基因序列分析[J].动物医学进展,2018,39(4):19-23,5.基金项目
北京市农林科学院科技创新能力建设专项(KJCX20140404) （KJCX20140404）
