动物医学进展2018，Vol.39Issue(4)：42-48,7.
当归补血颗粒的质量标准研究
Study on Quality Standard of Danggui Buxue Keli
摘要
Abstract
In order to establish the quality detection standard of Danggui Buxue Keli,ligustilide,the effective constituent of Angelica sinensis in the prescription,was identified by thin-layer chromatography (TLC), and the method of HPLC for the detection of ferulic acide,astragaloside,the effective constituents of An-gelica sinensis and Astragalus membranaceus was studied.The results showed that the dots of TLC were clear,separation degree was good,specificity was strong.The ferulic acid had a good linearity in the range of 0.502 μg/mL-20.06 μg/mL(R2=0.999 5),the precision RSD of 3 concentrations of ferulic acid was no more than 1.5%,repeatability test RSD was 0.63%,stability test RSD was 0.93%,the average recovery rate was 98.21%.The negative control had no interference,the specificity was good.The astragaloside had a good linearity in the range of 0.401 μg/mL-8.016 μg/mL(R2=0.999 2),the precision RSD of 3 concentra-tions of astragaloside was no more than 1.5%,repeatability test RSD was 1.76%,stability test RSD was 1. 66%,the average recovery rate was 97.68%.The negative control had no interference,the specificity was good.The detection method established in this study is simple,accurate,specific and reproducible,which can effectively control the quality of Danggui Buxue Keli.关键词
当归补血颗粒/薄层色谱法/高效液相色谱法/质量检测Key words
Danggui Buxue Keli/TLC/HPLC/quality detection分类
农业科技引用本文复制引用
李思聪,李旭廷,李金良,张敏,王斌,杨锐..当归补血颗粒的质量标准研究[J].动物医学进展,2018,39(4):42-48,7.基金项目
四川省财政运行专项项目(SASA2014CZYX010) （SASA2014CZYX010）
四川省科技支撑计划项目(15NC0018) （15NC0018）
