动物营养学报2018，Vol.30Issue(5)：1681-1692,12.DOI:10.3969/j.issn.1006-267x.2018.05.011
饲粮豆粕水平与β-甘露聚糖酶对断奶仔猪血清半乳甘露聚糖含量、生化指标及肠道氨基酸转运载体基因表达的影响
Effects of Dietary Soybean Meal Level and β-Mannanase on Serum Galactomannan Content, Biochemical Indices and Intestinal Amino Acid Transporters Gene Expression of Weaner Piglets
摘要
Abstract
According to the analysis of α-galactomannan(α-GM), β-galactomannan(β-GM)and galacto-mannan(GM)contents in 22 kinds of feed ingredients, this study evaluated the effect of β-mannanase (β-MN)on the contents of α-GM, β-GM and GM in serum, serum biochemical indices, and the gene rela-tive expression of solute carrier family 7 member 1(SLC7A1),solute carrier family 7 member 11(SLC7A11) and solute carrier family 38 member 2(SLC38A2)of weaner piglets. Twenty-four healthy dualistic hybrid weaner piglets with similar body weight were randomly divided into 4 groups(groupsⅠ,Ⅱ,ⅢandⅣ)with 6 replicates per group and 1 pig per replicate in a 2×2 factorial design. Piglets in groupⅠfed the 22% soybean meal(SBM)level diet, piglets in group Ⅱ fed the 22% SBM level diet supplemented with 0.02% β-MN, piglets in groupⅢfed the 37% SBM level diet,and the others in groupⅣfed the 37% SBM level diet supple-mented with 0.02% β-MN. The experiment lasted for 30 days. At day 30,10 mL blood samples from carotid artery, mesenteric vein and portal vein were collected from 24 piglets for serum α-GM, β-GM and GM con-tents determination,respectively;10 mL blood from precaval vein used for serum biochemical indices analysis. The proximal jejunum and distal ileum samples were collected for analysis gene relative expression of SLC7A1, SLC7A11 and SLC38A2. The results showed that compared with groupⅠ,the average daily feed intake(AD-FI)and SLC7A1 gene relative expression in ileum of weaner piglets in group Ⅲ were significantly decreased (P<0.05), the average daily intake of α-GM content, the content of β-GM in carotid artery serum, the con-tents of β-GM and GM in mesenteric vein serum,the alanine aminotransferase,glutamic oxaloacetic transami-naseand activities and urea nitrogen content in precaval vein serum of weaner piglets in group Ⅲ were signifi-cantly increased(P<0.05); the content of α-GM in mesenteric vein serum,the contents of α-GM and GM in portal vein serum of weaner piglets in group Ⅱ were significantly decreased(P<0.05), the contents of glu-cose, calcium and high density lipoprotein in precaval vein serum were significantly increased(P<0.05). Compared withⅢgroup,the content of GM in portal vein serum of weaner piglets in groupⅣwas significant-ly decreased(P<0.05), the gene relative expressions of SLC7A1, SLC7A11 and SLC38A2 in jejunum were significantly increased(P<0.05). These findings suggest that with the average daily intake of α-GM content increase, the ADFI is decreased; the contents of α-GM, β-GM, and GM in carotid artery, mesenteric vein, and hepatic portal vein serum are increased by the dietary α-GM,β-GM and GM contents increase. When diet-ary α-GM, β-GM and GM increase, β-MN can decrease the content of GM in portal vein serum and increase the gene relative expression of SLC7A1,SLC7A11 and SLC38A2 in jejunum.关键词
β-甘露聚糖酶/半乳甘露聚糖/豆粕/氨基酸转运载体/生化指标/断奶仔猪Key words
β-mannanase/galactomannan/soybean meal/amino acid transporter/biochemical indices/weaner piglets分类
农业科技引用本文复制引用
朱晓彤,张方亮,尹杰,黎育颖,韩慧,宾石玉,李铁军,印遇龙..饲粮豆粕水平与β-甘露聚糖酶对断奶仔猪血清半乳甘露聚糖含量、生化指标及肠道氨基酸转运载体基因表达的影响[J].动物营养学报,2018,30(5):1681-1692,12.基金项目
国家973计划课题(2013CB127301) （2013CB127301）
礼来动物保健基金(ELACN150334) （ELACN150334）
